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| Status |
Public on Sep 01, 2023 |
| Title |
A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia (bulk ATAC-seq data from ganglioid cultures) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Glial cells have been proposed as an endogenous source of progenitors for the treatment of neural deficits. However, the cellular and molecular mechanisms underpinning the neurogenic potential of certain populations of adult glial cells, are not known. Using single cell transcriptomic profiling, we show here that enteric glial cells represent a cell state attained by autonomic neural crest cells as they transition during development along a linear default differentiation trajectory that allows them to retain neurogenic potential while acquiring a gene expression profile associated with their role in neuronal support and immunomodulation. Key neurogenic loci in early enteric nervous system progenitors remain in open chromatin configuration in mature enteric glia, thus facilitating neuronal differentiation under appropriate conditions. Molecular profiling and gene targeting of enteric glial cells in a novel cell culture system of enteric neurogenesis and a gut injury model, demonstrated that neuronal differentiation of glia is driven by transcriptional programs employed in vivo by early progenitors. Our work provides mechanistic insight into the dynamic regulatory landscape underpinning the development of intestinal neural circuits and generates a platform for advancing glial cells as therapeutic agents for the treatment of neural deficits.
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| Overall design |
We performed bulk ATAC-seq of the tdT+ descendants of enteric glial cells from DIV4 ganglioids.
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| Contributor(s) |
Laddach A, Chng SH, Lasrado R, Progatzky F, Shapiro M, Erickson A, Sampedro-Castaneda M, Artemov A, Bon-Frauches AC, Amaniti E, Kleinjung J, Boeing S, Ultanir S, Adameyko I, Pachnis V |
| Citation(s) |
37737269 |
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| Submission date |
Aug 23, 2023 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Vassilis Pachnis |
| E-mail(s) |
Vassilis.Pachnis@crick.ac.uk
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| Phone |
+442037961556
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| Organization name |
The Francis Crick Institute
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| Department |
Development and Homeostasis of the Nervous System Laboratory
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| Lab |
Pachnis
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| Street address |
1 Midland Road
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| City |
London |
| State/province |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1008579 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE241522_DIV4_60.mSm_peaks.annotatePeaks.txt.gz |
2.4 Mb |
(ftp)(http) |
TXT |
| GSE241522_DIV4_60.mSm_peaks.broadPeak.gz |
2.3 Mb |
(ftp)(http) |
BROADPEAK |
| GSE241522_DIV4_60.mSm_peaks.gappedPeak.gz |
3.1 Mb |
(ftp)(http) |
GAPPEDPEAK |
| GSE241522_DIV4_60.mSm_peaks.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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