 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 18, 2024 |
| Title |
P300 Regulates Histone Crotonylation and Preimplantation Embryo Development (ChIP-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Histone lysine crotonylation is a non-acetyl histone lysine modification that is evolutionarily conserved. It plays an important role in regulating various biological processes, including gene transcriptional regulation, spermatogenesis, and cell cycle. However, the dynamic changes and functions of histone crotonylation in preimplantation embryonic development in mammals remain unclear. In this study, we have shown that the transcription coactivator P300 functions as a writer of histone crotonylation during embryonic development. Depletion of P300 resulted in significant developmental defects and dysregulation of the transcriptome of embryos. Importantly, we demonstrated that P300 catalyzes the crotonylation of histone, directly stimulating transcription and regulating gene expression, thereby ensuring successful embryo development to the blastocyst stage. Furthermore, it is showed that the histone H3 lysine 18 crotonylation (H3K18cr) modification is predominantly located in active promoter regions. It serves as an epigenetic hallmark of key transcriptional regulators and promotes the transcription activation of genes. Together, our results propose a model wherein P300-mediated histone crotonylation plays a crucial role in regulating the fate of embryonic development.
|
| |
|
| Overall design |
Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone H3 lyisne 18 sites crotonylation modifications (H3K18cr) in preimplantation embryos.
|
| |
|
| Contributor(s) |
Gao D, Li C, Liu S, Xu T, Lin X, Tan Y, Gao F, Yi L, Zhang JV, Ma J, Meng T, Yeung WB, Liu K, Ou X, Su R, Sun Q |
| Citation(s) |
39080296 |
| |
| Submission date |
Aug 18, 2023 |
| Last update date |
Aug 26, 2024 |
| Contact name |
Di Gao |
| E-mail(s) |
gaodi8808695@gmail.com
|
| Phone |
18715047780
|
| Organization name |
the University of Hong Kong Shenzhen Hospital
|
| Street address |
No. 1, Haiyuan 1st Road, Futian District
|
| City |
Shenzhen |
| ZIP/Postal code |
518053 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (22)
|
| GSM7719145 |
Control H3K18cr Morula rep2 ChIP |
| GSM7719146 |
Control H3K18cr Late-8-cell rep1 ChIP |
| GSM7719147 |
Control H3K18cr Late-8-cell rep2 ChIP |
| GSM7719148 |
Control POL(II) Late-8-cell rep1 ChIP |
| GSM7719149 |
Control POL(II) Late-8-cell rep2 ChIP |
| GSM7719150 |
P300 siRNA H3K18cr Late-8-cell rep1 ChIP |
| GSM7719151 |
P300 siRNA H3K18cr Late-8-cell rep2 ChIP |
| GSM7719152 |
P300 siRNA I1394G H3K18cr Late-8-cell rep1 ChIP |
| GSM7719153 |
P300 siRNA I1394G H3K18cr Late-8-cell rep2 ChIP |
| GSM7719154 |
P300 siRNA NaCr H3K18cr Late-8-cell rep1 ChIP |
| GSM7719155 |
P300 siRNA NaCr H3K18cr Late-8-cell rep2 ChIP |
| GSM7719156 |
P300 siRNA POL(II) Late-8-cell rep1 ChIP |
| GSM7719157 |
P300 siRNA POL(II) Late-8-cell rep2 ChIP |
| GSM8154269 |
Control H3K27ac Late-2-cell rep1 ChIP |
| GSM8154270 |
Control H3K27ac Late-2-cell rep2 ChIP |
| GSM8154271 |
Control H3K4me3 Late-8-cell rep1 ChIP |
| GSM8154272 |
Control H3K4me3 Late-8-cell rep2 ChIP |
| GSM8154273 |
P300 siRNA H3K4me3 Late-8-cell rep1 ChIP |
| GSM8154274 |
P300 siRNA H3K4me3 Late-8-cell rep2 ChIP |
|
| This SubSeries is part of SuperSeries: |
| GSE241196 |
P300 Regulates Histone Crotonylation and Preimplantation Embryo Development |
|
| Relations |
| BioProject |
PRJNA1006717 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE241193_RAW.tar |
5.7 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...