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| Status |
Public on Aug 01, 2024 |
| Title |
Multi-omic map revealed PPARĪ± activation protecting against myocardial ischemia/reperfusion injury by maintaining cardiac metabolic homeostasis [snRNA-seq] |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The rats were subjected to left anterior descending (LAD) coronary artery ligation for 1 hour, 6 hours (MI1h and MI6h) and 24 hours (MI24h) along with Sham controls (Sham), and 1-hour, 6-hour ischemia followed by reperfusion (MI1h/R and MI6h/R, mimicking early time reperfusion (ETR) and late time reperfusion (LTR), respectively) followed by heart tissue collection 24 hours post MI to mimic the MI/R injury in AMI patients.The heart infarct zones (Sham, MI1h/R, MI6h/R and MI24h) were isolated to single-nucleus transcriptome analysis. Collected nuclei for each sample were loaded onto 10X Chrominum platform. We obtained 22,234 cells and 17,003 genes. Five major cell types were identified, which included cardiomyocytes (CMs), endothelial cells (ECs), fibroblasts (FBs), macrophages (MACs) and smooth muscle cells (SMCs).
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| Overall design |
Single-nucleus RNA sequencing performed with rats subjected to left anterior descending (LAD) coronary artery ligation for 1 hour, 6 hours (MI1h and MI6h) and 24 hours (MI24h) along with Sham controls (Sham), and 1-hour, 6-hour ischemia followed by reperfusion (MI1h/R and MI6h/R, mimicking early time reperfusion (ETR) and late time reperfusion (LTR), respectively) followed by heart tissue collection 24 hours post MI to mimic the MI/R injury in AMI patients.
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| Contributor(s) |
Gao Y, Huang F, Ruan F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Aug 15, 2023 |
| Last update date |
Aug 01, 2024 |
| Contact name |
Li Shen |
| E-mail(s) |
shenlab@zju.edu.cn
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| Phone |
86-0571-88981751
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| Organization name |
Life Sciences Institute, Zhejiang University
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| Lab |
Shenlab
|
| Street address |
866 Yuhangtang Road
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| City |
Hangzhou |
| State/province |
Zhejiang Province |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE240850 |
Multi-omic map revealed PPARĪ± activation protecting against myocardial ischemia/reperfusion injury by maintaining cardiac metabolic homeostasis. |
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| Relations |
| BioProject |
PRJNA1005555 |
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