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| Status |
Public on May 31, 2024 |
| Title |
Longitudinal biopsies of fusion-positive rhabdomyosarcoma orthotopic xenografts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this experiment, 3 mice were implanted orthotopically with a patient-derived xenograft, SJRHB013759_X14. After tumor engraftment and visible nodule formation, all 3 mice underwent therapy. One mouse received vehicle control (saline), while two mice were treated with a combination of vincristine and irinotecan, to mimic salvage therapy for relapsed rhabdomyosarcoma. Sedated needle biopsies were performed before (time = 0), during therapy (time = 3, 7, and 14 days), and after recurrence.
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| |
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| Overall design |
Mice bearing orthotopic xenografts underwent sedated biopsies before, during and after completion of therapy. Tissue biopsies underwent Trizol RNA extraction, followed by automated RNA-sequencing library construction.
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| Contributor(s) |
Patel A, Stewart E, Dyer M |
| Citation(s) |
39060228 |
| |
| Submission date |
Aug 07, 2023 |
| Last update date |
Aug 30, 2024 |
| Contact name |
Michael Dyer |
| Organization name |
St. Jude Children's Research Hospital
|
| Department |
Developmental Neurobiology
|
| Street address |
262 Danny Thomas Pl MS 324
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA1003123 |
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