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| Status |
Public on Sep 01, 2023 |
| Title |
Transcriptional differences in breast cancer cells exposed to acute versus chronic hypoxia. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our previous studies demonstrated that exposing cells to acute hypoxic conditions in vitro (24 h at 1% oxygen) does not fully recapitulate exposure to intratumoral hypoxia (Godet et al., 2019). In this study, we sought to investigate whether chronic exposure to hypoxia in vitro (10 days at 1% oxygen) would better mimic the transcriptional effects of intratumoral hypoxia.
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| Overall design |
To compare acute versus chronic hypoxic conditions, MDA-MB-231 cells were cultured for 1 or 10 days under 20% or 1% O2. To investigate the effect of reoxygenation, cells cultured for 10 days under 1% O2 were then subjected to 10 days under 20% O2. RNA was extracted using the Direct-zol miniRNA kit (Zymo) and samples with RIN>9 were submitted for library preparation, RNA sequencing and preliminary analysis.
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| Contributor(s) |
Godet I, Gilkes D |
| Citation(s) |
39341835 |
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| Submission date |
Aug 07, 2023 |
| Last update date |
Oct 15, 2024 |
| Contact name |
Ines Godet |
| Organization name |
Johns Hopkins University
|
| Department |
Oncology
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| Lab |
Gilkes
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| Street address |
1650 Orleans Street, CRB1, room 128
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21231 |
| Country |
USA |
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| Platforms (1) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA1002981 |
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