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| Status |
Public on Oct 26, 2023 |
| Title |
Haploid androgenetic development of bovine embryos reveals imbalanced WNT signaling and impaired cell fate differentiation. |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Haploid embryos have contributed significantly to our understanding of the role of parental genomes in development and can be applied to important biotechnology for human and animal species. However, development to the blastocyst stage is severely hindered in bovine haploid androgenetic embryos (hAE). To further our understanding of such developmental arrest, we performed a comprehensive comparison of the transcriptomic profile of morula-stage embryos, which were validated by qRT-PCR of transcripts associated with differentiation in haploid and biparental embryos. Among numerous disturbances, results showed that pluripotency pathways, especially the wingless-related integration site (WNT) signaling, were particularly unbalanced in hAE. Moreover, transcript levels of KLF4, NANOG, POU5F1, SOX2, CDX2, CTNNBL1, AXIN2, and GSK3B were noticeably altered in hAE, suggesting disturbance of pluripotency and canonical WNT pathways. To evaluate the role of WNT on hAE competence, we exposed early day-5 morula stage embryos to the GSK3B inhibitor CHIR99021. Although no alterations were observed in pluripotency and WNT-related transcripts, exposure to CHIR99021 improved their ability to reach the blastocysts stage, confirming the importance of the WNT pathway in the developmental outcome of bovine hAE.
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| Overall design |
Bovine embryos at morula stage being Haploid Androgenotes, Haploid Parthenote and ICSI.
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| Web link |
https://doi.org/10.1093/biolre/ioad124
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| Contributor(s) |
Nociti RP, Paredes LA, Smith LC |
| Citation(s) |
37788061 |
| |
| Submission date |
Aug 02, 2023 |
| Last update date |
Oct 26, 2023 |
| Contact name |
Ricardo Perecin Nociti |
| E-mail(s) |
rnociti@usp.br, ricardo.perecin.nociti@umontreal.ca
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| Organization name |
University of Sao Paulo
|
| Department |
Department of Veterinary Medicine - ZMV
|
| Lab |
Laboratory of Molecular Morphophysiology and Development - LMMD
|
| Street address |
Av. Duque de Caxias Norte, 225 Campus Fernando Costa – USP
|
| City |
Pirassununga |
| State/province |
Sao Paulo |
| ZIP/Postal code |
13635-900 |
| Country |
Brazil |
| |
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| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA1001351 |
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