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| Status |
Public on Dec 27, 2023 |
| Title |
Transcriptome analysis for differentiated gene expression analysis of TU2218, Vactosertib, and Ramucirumab on VEGF-induced endothelial cell anergy condition |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptome analysis of HUVECs treated with TGFβRI or VEGFR2 inhibitors on endothelial cell anergy condition
In this study, the dual inhibitory mechanism of TU2218 was identified using an in vitro analysis mimicking the tumor microenvironment and its anti-tumor effects were analyzed using in vivo mouse syngeneic tumor models. TU2218 directly normalized the activity of damaged T lymphocytes and natural killer cells from TGFβ and suppressed the activity and viability of regulatory T cells. The anergy of endothelial cells induced by VEGF stimulation was completely ameliorated by TU2218, an effect not observed with vactosertib, which only inhibits TGFβ signal.
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| Overall design |
18 RNA samples including triplicate-repeated samples for 6 conditions using the Affymetrix Human 2.0 ST platform. The data were summarized and normalized with robust multi-average (RMA) method implemented in Affymetrix® Power Tools (APT). We exported the result with gene level RMA analysis and performed the differentially expressed gene (DEG) analysis.
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| Contributor(s) |
Kim N, Lee J, Kang S, Kim S, Bae S, Kim H |
| Citation(s) |
39105882 |
| |
| Submission date |
Aug 01, 2023 |
| Last update date |
Sep 05, 2024 |
| Contact name |
Jihyun Lee |
| E-mail(s) |
ljh2276@gmail.com
|
| Phone |
+821046325268
|
| Organization name |
Tiumbio
|
| Department |
Discovery2
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| Street address |
30, Changeop-ro 40beon-gil, Sujeong-gu
|
| City |
Seongnam-si, Gyeonggi-do |
| ZIP/Postal code |
13449 |
| Country |
South Korea |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA1000926 |
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