 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 11, 2023 |
| Title |
Hormone responsiveness and spatial heterogeneityof apical-out endometrial organoids |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have developed apical-out (AO)- endometrial organoids (EMO) that emulate the in vivo archtecture of endometrial epithelium. The AO-EMO exposes the apical surface of the epithelium. To explore the hormone responsiveness and spatial heterogeneity of AO-EMO, we conducted transcriptomic analysis.
|
| |
|
| Overall design |
We cultured human endometrial organoids in collagen-based gels (70% type-I collagen and 30% matrigel) for 7-10 days with ECSY medium. The composition of the ECSY medium was as follows: DMEM/F-12 supplemented with 1% ITS-X supplement, 0.15% BSA, 1% Knockout Serum Replacement, 200 mM L-ascorbic acid, 50 ng/ml EGF, 2 μM CHIR99021, 2 μM SB202190, and 10 μM Y27632. Following the ECSY culture, we sequentially introduced 17β-estradiol(E2), medroxyprogesterone acetate (MPA), 8-bromoadenosine 3′,5′-cyclic monophosphate (8-Br-cAMP), and prolactin. The treatment durations were as follows: 2 days for E2 and 4 days for the combined treatment of E2, MPA, 8-Br-cAMP, and prolactin (EPCP). As a control, we prepared AO-EMO without any hormone treatment as well as AO-EMO treated exclusively with E2. To isolate the outer and inner cells of AO-EMO, the culture medium was aspirated, and PBS was added to wash. Subsequently, TrypLE Express was added to the culture dish, and plates were incubated at 37℃ for 30 min. Following the incubation, TrypLE was removed and AO-EMO were washed with PBS. The outer cells were detached by pipetting PBS onto the surface of the AO-EMO several times using a manual pipette. We used 4 independent organoids from 4 donors.
|
| |
|
| Contributor(s) |
Shibata S, Endo S, Arima T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 31, 2023 |
| Last update date |
Nov 11, 2023 |
| Contact name |
Shun Shibata |
| E-mail(s) |
shun.shibata.a3@tohoku.ac.jp
|
| Organization name |
Tohoku University
|
| Department |
Graduate School of Medicine
|
| Lab |
Informative Genetics
|
| Street address |
2-1 Seiryo-chou
|
| City |
Sendai |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (22)
|
|
| Relations |
| BioProject |
PRJNA1000494 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE239657_Count_AOEMO_InOut.csv.gz |
676.6 Kb |
(ftp)(http) |
CSV |
| GSE239657_TPM_AOEMO_InOut.csv.gz |
872.9 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...