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| Status |
Public on Sep 27, 2023 |
| Title |
Tumor-infiltrating CCR2+ inflammatory monocytes counteract specific immunotherapy |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used Single-cell RNA-sequencing to analyze the composition of the tumor microenvironment of MC38mOVA tumors upon transcutaneous immunization with our immunization method DIVA.
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| Overall design |
MC38mOVA tumor cells were inoculated s.c. into WT mice. Mice were treated by transcutaneous immunization at day 6/7 and 13/14 after inoculation or left untreated. At day 16, respectively day 20, single cell suspensions were prepared from excised whole tumors, and CD45+ cells were enriched by magnetic bead separation. Afterwards Single-cell RNA-sequencing of these CD45+ cells was performed.
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| Web link |
http://doi: 10.3389/fimmu.2023.1267866
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| Contributor(s) |
Hartmann A, Arnold-Schild D, Stein L, Klein M, Marini F |
| Citation(s) |
37849753 |
| |
| Submission date |
Jul 27, 2023 |
| Last update date |
Oct 26, 2023 |
| Contact name |
Matthias Klein |
| E-mail(s) |
drmklein@uni-mainz.de
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| Organization name |
University Medical Center Mainz
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| Department |
Inst. for Immunology
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| Street address |
Langenbeckstrasse 1
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| City |
Mainz |
| ZIP/Postal code |
55131 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
| GSM7679699 |
mixed sample of MC38mOVA_Control_DIVA2treated_day16 |
| GSM7679700 |
mixed sample of MC38mOVA_Control_DIVA2treated_day20 |
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| Relations |
| BioProject |
PRJNA1001913 |
