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Series GSE237838 Query DataSets for GSE237838
Status Public on Sep 11, 2023
Title IL-1b+ tumor-associated macrophages fuel pathogenic inflammation in pancreatic cancer - Bulk_mouse_invitro
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease with high resistance to therapy. Inflammatory and immunomodulatory signals co-exist in the tumor microenvironment (TME), leading to dysregulated reparative and cytotoxic responses. Tumor-associated macrophages (TAMs) control immune dynamics in the TME, but their heterogeneity and plasticity have hampered understanding of the underlying mechanisms. Here, we combined single-cell and spatial genomics with functional experiments to elucidate macrophage functions in PDAC. We uncovered an inflammatory loop between tumor cells and interleukin (IL)-1b+ TAMs, a subset of macrophages elicited by a local synergy between prostaglandin E2 (PGE2) and tumor necrosis factor (TNF)-a. Interfering with the PGE2-IL-1b axis elicited TAM reprogramming and antagonized tumor-intrinsic inflammation, leading to PDAC control in vivo. IL-1b+ TAMs are conserved across human cancers and correlate with inflammation and patient prognosis in a context-dependent manner. Our study highlights how TAMs govern between inflammation and immune suppression in cancer, with significant therapeutic implications.
 
Overall design Bulk RNA-seq of KC and KPC cell line in 2D and 3D cultures upon IL1-b stimulation.
 
Contributor(s) Caronni N, La Terza F, Vittoria FM, Barbiera G, Genua M, Ostuni R
Citation(s) 37914939
Submission date Jul 20, 2023
Last update date Nov 10, 2023
Contact name Renato Ostuni
Organization name San Raffaele Telethon Institute for Gene Therapy
Street address Via Olgettina 58
City Milano
ZIP/Postal code 20132
Country Italy
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (55)
GSM7655166 K8484 KPC cell line Il1b stimulation 24h RNA-seq replicate 1
GSM7655167 K8484 KPC cell line Il1b stimulation 24h RNA-seq replicate 2
GSM7655168 K8484 KPC cell line control 24h RNA-seq replicate 1
This SubSeries is part of SuperSeries:
GSE217847 IL-1b+ tumor-associated macrophages fuel pathogenic inflammation in pancreatic cancer
Relations
BioProject PRJNA996915

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE237838_K8484_DT6606_counts_matrix.csv.gz 1.1 Mb (ftp)(http) CSV
GSE237838_Organoids_KPC_counts_matrix.csv.gz 801.0 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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