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Series GSE237792 Query DataSets for GSE237792
Status Public on May 17, 2024
Title Transcriptome analysis of VapC13 and VapC26 overexpression in Mycobacterium tuberculosis
Organism Mycobacterium tuberculosis H37Rv
Experiment type Expression profiling by high throughput sequencing
Summary Using Total RNA sequencing, We report the effects of VapC13 and VapC26 overexpression on the transcriptome of M. tuberculosis. For RNA-seq experiments, early-log phase cultures (OD600nm ~ 0.2-0.3) of M. tuberculosis strains harboring either pTetR or pTetR-vapC13 or pTetR-vapC26 were induced with the addition of 50 ng/ml Atc for 24 h. For total RNA isolation, induced cultures were harvested by centrifugation, washed twice with 1x PBS and lysed by bead beating in Trizol. Total RNA was extracted by phenol-chloroform, precipitated with isopropanol and eluted using Qiagen RNA isolation kit as per manufacturer’s instructions. We report that VapC13 or VapC26 resulted in increased transcripts levels of stress responsive genes of M. tuberculosis.
Overall design Cells harbouring pTetR (Vector only) or pTetR VapC13 or pTetR VapC26 were used for RNA-Seq analysis. Experiments were performed thrice for each strain.
Contributor(s) Sharma A, Singh N, Bhasin M, Tiwari P, Chopra P, Vardarajan R, Singh R
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Submission date Jul 19, 2023
Last update date May 17, 2024
Contact name Munmun Bhasin
Organization name Indian Institute of Science
Department Molecular Biophysics Unit
Street address CV Raman Rd
City Bangalore
State/province Karnataka
ZIP/Postal code 560012
Country India
Platforms (1)
GPL29178 Illumina NovaSeq 6000 (Mycobacterium tuberculosis H37Rv)
Samples (9)
GSM7653386 pTetR Control Replicate 1, 24 hours
GSM7653387 pTetR Control Replicate 2, 24 hours
GSM7653388 pTetR Control Replicate 3, 24 hours
BioProject PRJNA996620

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Supplementary file Size Download File type/resource
GSE237792_VapC13_overexpression.txt.gz 138.7 Kb (ftp)(http) TXT
GSE237792_VapC26_overexpression.txt.gz 143.7 Kb (ftp)(http) TXT
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