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| Status |
Public on Sep 14, 2023 |
| Title |
Phage-Antibiotic Combinations Against Klebsiella pneumoniae |
| Organism |
Klebsiella pneumoniae subsp. pneumoniae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
hvKP ATCC43816 and its lytic phage H5 were employed as a phage-antibiotic combination model. Based on the comprehensive characterization of phages, including cryo-electron microscopy, we evaluated the synergic effect of H5 on bacterial killing in vitro when combined with multiple antibiotics, and analyzed the advantages of phage-antibiotic combinations from an evolutionary perspective and proposes a novel PAS mechanism by using ceftazidime as an example.
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| Overall design |
To investigate the underlying resistance mechanisms of Kpn 43816 against single phage stress and phage-antibiotic combination stress, we performed RNA-Seq analysis on four strains under different stress conditions: CP17, which is resistant to the phage-antibiotic combination but shows no mutations in the genome; CP6, which is resistant to the phage-antibiotic combination and contains a wcaJ mutation; P2 and P13, which are resistant to phage only and contain wcaJ mutations; and wild-type strains.
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| Contributor(s) |
Zhou K, Qin K, Shi X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 17, 2023 |
| Last update date |
Sep 14, 2023 |
| Contact name |
xing shi |
| E-mail(s) |
shixinggl@gmail.com
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| Organization name |
Shenzhen Institute of Respiratory Disease
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| Department |
Department of Respiratory and Critical Care Medicine
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| Street address |
Luohu
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| City |
Shenzhen |
| State/province |
Guangdong |
| ZIP/Postal code |
518055 |
| Country |
China |
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| Platforms (1) |
| GPL30661 |
Illumina NovaSeq 6000 (Klebsiella pneumoniae subsp. pneumoniae) |
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| Samples (15)
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| GSM7622151 |
Kpn 43816 CP17, replicate 1 |
| GSM7622152 |
Kpn 43816 CP17, replicate 2 |
| GSM7622153 |
Kpn 43816 CP17, replicate 3 |
| GSM7622154 |
Kpn 43816 CP6, replicate 1 |
| GSM7622155 |
Kpn 43816 CP6, replicate 2 |
| GSM7622156 |
Kpn 43816 CP6, replicate 3 |
| GSM7622157 |
Kpn 43816 P2, replicate 1 |
| GSM7622158 |
Kpn 43816 P2, replicate 2 |
| GSM7622159 |
Kpn 43816 P2, replicate 3 |
| GSM7622160 |
Kpn 43816 P13, replicate 1 |
| GSM7622161 |
Kpn 43816 P13, replicate 2 |
| GSM7622162 |
Kpn 43816 P13, replicate 3 |
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| Relations |
| BioProject |
PRJNA995503 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE237490_raw_counts.txt.gz |
143.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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