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| Status |
Public on Jul 12, 2023 |
| Title |
Single-Cell Analysis of Dorsal Root Ganglia Reveals Metalloproteinase Signaling in Satellite Glial Cells and Pain |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Although satellite glial cells (SGCs) are among the most abundant non-neuronal cells in dorsal root ganglia (DRG), little is known about their heterogenity and functions. We used single cell RNA sequencing (scRNA-seq) to analyze the heterogenity and unique functions of SGCs.
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| Overall design |
DRG tissues from three CD-1 mice were dissected and pooled, single cell suspension was generated by enzymatic and mechanical dissociation, and analyzed using scRNA-seq.
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| Contributor(s) |
Berta T, Tonello T, Paranjpe A |
| Citation(s) |
37557960 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 NS113243 |
Targeting sensory ganglia and glial signaling for the treatment of acute and chronic pain |
UNIVERSITY OF CINCINNATI |
Temugin Berta |
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| Submission date |
Jul 10, 2023 |
| Last update date |
Aug 31, 2023 |
| Contact name |
Temugin Berta |
| E-mail(s) |
temugin.berta@uc.edu
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| Organization name |
University of Cincinnati
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| Department |
Anesthesiology
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| Street address |
231 Albert Sabin Way, MSB 3408
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| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45267 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA993119 |
