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| Status |
Public on Aug 19, 2010 |
| Title |
RNA immunoprecipitation to identify RHA-binding transcripts in HEK293 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Given that RHA regulates translation by binding a PCE located at the 5’ UTR of the target transcripts a genome-wide screen was performed to identify mRNAs that bind RHA in vivo. The results from four experiments with samples obtained in four independent RNA immunoprecipitations identified 375 transcripts that co-immunoprecipitate with FLAG RHA.
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| Overall design |
Since N-terminal FLAG tagged RHA specifically co-immunoprecipitates PCE-containing mRNAs HEK293 cells were transfected with a CMV-FLAG-RHA construct. Cytoplasmic lysates were immunoprecipitated with anti-FLAG beads. RNA was extracted from the immunoprecipitate and used to probe a human Agilent expression arrays. An immunoprecipitation with cells transfected with empty FLAG plasmid was used as negative control.
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| Contributor(s) |
Boris-Lawrie K, Hernandez JM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 18, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Kathleen Boris-Lawrie |
| E-mail(s) |
Kathleen.Boris-Lawrie@cvm.osu.edu
|
| Phone |
(614) 292-1392
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| Fax |
(614) 292-6473
|
| URL |
http://www.vet.ohio-state.edu/KathleenBoris-Lawrie.htm
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| Organization name |
The Ohio State University
|
| Department |
Department of Veterinary Biosciences
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| Street address |
1925 Coffey Rd
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE23689 |
Identification of RNA helicase A translation target mRNAs at the intersection of RNA precipitation and polysome arrays |
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| Relations |
| BioProject |
PRJNA133139 |
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