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| Status |
Public on Mar 19, 2024 |
| Title |
IL-33 controls IL-22-dependent antibacterial defense by modulating the microbiota [shotgun sequencing] |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
IL-22 plays a critical role in defending against mucosal infections, but how IL-22 production is regulated is incompletely understood. Here, we show that mice lacking IL-33 or its receptor ST2 (IL-1RL1) were more resistant to Streptococcus pneumoniae lung infection than wild-type animals, and that single nucleotide polymorphisms in IL33 and IL1RL1 were associated with pneumococcal pneumonia in humans. The effect of IL-33 on S. pneumoniae infection was mediated by negative regulation of IL-22 production by innate lymphoid cells (ILCs), but independent of ILC2s as well as IL-4 and IL-13 signaling. Moreover, IL-33´s influence on antibacterial defense was dependent on housing conditions of the mice, and mediated by the modulatory effect of IL-33 on the intestinal microbiota. Collectively, we reveal that IL-33 controls IL-22-dependent antibacterial defense by modulating the microbiota. Our study thus provides insight into the bidirectional crosstalk between the innate immune system and the microbiota and how it shapes susceptibility to bacterial infection.
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| Overall design |
Shotgun sequencing of 65 stool samples of infected mice from different vivaria
Sample_list.xlsx is necessary for the R script to reproduce the analysis
Please note that raw data is unavailable as fastq files have been deleted.
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| Contributor(s) |
Röwekamp I, Opitz B |
| Citation(s) |
38781213 |
| |
| Submission date |
Jul 03, 2023 |
| Last update date |
Jun 18, 2024 |
| Contact name |
Bastian Opitz |
| Organization name |
Charité – Universitätsmedizin Berlin
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| Department |
Department of Infectious Diseases, Respiratory Medicine and Critical Care
|
| Street address |
Augustenburgerplatz 1
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| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (65)
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| This SubSeries is part of SuperSeries: |
| GSE236344 |
IL-33 controls IL-22-dependent antibacterial defense by modulating the microbiota. |
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| Relations |
| BioProject |
PRJNA990667 |
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