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| Status |
Public on Aug 07, 2024 |
| Title |
CRISPR Screen: The Interferon γ Pathway Enhances Pluripotency and X-Chromosome Reactivation in iPSC Reprogramming |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
To investigate the genes and pathways involved in X-chromosome reactivation and pluripotency acquisition, we generated a whole-genome CRISPR knockout screen during female NPC reprogramming into iPSCs. We analyzed the gRNA abundance in NPCs and three different reprogramming populations: non-pluripotent, early pluripotent and late pluripotent. Pair-wise comparisons of gRNA frequencies in different populations were done to identify genes and pathways involved in cell survival in reprogramming conditions, colony formation, early pluripotency acquisition and late pluripotency/X-chromosome reactivation.
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| |
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| Overall design |
DNA sequencing of CRISPR-gRNA libraries in NPCs and cells undergoing reprogramming with different pluripotency and X-chromosome status.
|
| Web link |
http://www.science.org/doi/10.1126/sciadv.adj8862
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| |
|
| Contributor(s) |
Barrero M, Payer B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 30, 2023 |
| Last update date |
Aug 07, 2024 |
| Contact name |
Mercedes Barrero |
| Organization name |
Centre for Genomic Regulation
|
| Street address |
Dr. Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (8)
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| GSM7520276 |
gRNA sequencing - Non-Pluripotent Biol Rep2 |
| GSM7520277 |
gRNA sequencing - Early Pluripotent Biol Rep1 |
| GSM7520278 |
gRNA sequencing - Early Pluripotent Biol Rep2 |
| GSM7520279 |
gRNA sequencing - Late Pluripotent Biol Rep1 |
| GSM7520280 |
gRNA sequencing - Late Pluripotent Biol Rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE236247 |
The Interferon γ Pathway Enhances Pluripotency and X-Chromosome Reactivation in iPSC Reprogramming |
|
| Relations |
| BioProject |
PRJNA989564 |
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