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| Status |
Public on Aug 18, 2024 |
| Title |
De-phosphorylation of bZIP59 by PP2A ensures appropriate shade avoidance response in Arabidopsis |
| Organism |
Arabidopsis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Shade can trigger the shade avoidance syndrome (SAS) in shade-intolerant species, which cause exaggerated growth and affect crop yield. We report that Arabidopsis transcription factors bZIP59 negatively regulate SAS. To identify direct targets of bZIP59 at the genome-wide level, we performed ChIP-Seq using ProbZIP59::bZIP59-GFP/bzip59 transgenic plants under white light or transferred to shade conditions for 2 hours. Our results indicated shade light dramatically increased the DNA binding ability of bZIP59, and shade-enhanced binding of bZIP59 majorly located around transcriptional start site (TSS) of genes.
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| Overall design |
7-day-old white-light-grown ProbZIP59::bZIP59-GFP/bzip59 seedlings were treated 2 hr shade before harvesting for DNA extraction.
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| Contributor(s) |
Li L, Li F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 29, 2023 |
| Last update date |
Aug 19, 2024 |
| Contact name |
Lin Li |
| E-mail(s) |
linli@fudan.edu.cn
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| Organization name |
Fudan university
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| Department |
school of life science
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| Street address |
2005 Songhu Road, Yangpu Distric
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| City |
Shanghai |
| ZIP/Postal code |
200438 |
| Country |
China |
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| Platforms (1) |
| GPL27784 |
Illumina NovaSeq 6000 (Arabidopsis) |
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| Samples (8)
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| GSM7518330 |
White light, Input, rep2 |
| GSM7518331 |
Shade, IP, rep1 |
| GSM7518332 |
Shade, Input, rep1 |
| GSM7518333 |
Shade, IP, rep2 |
| GSM7518334 |
Shade, Input, rep2 |
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| Relations |
| BioProject |
PRJNA989032 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE236101_RAW.tar |
405.9 Mb |
(http)(custom) |
TAR (of BW, NARROWPEAK) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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