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| Status |
Public on Dec 29, 2023 |
| Title |
Comparative analysis of the transcriptional response to bulky DNA damages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A systematic RNA-seq study comparing the transcriptional response to three independent bulky DNA damage inducing agents: UV, the chemotherapy cisplatin and benzo[a]pyrene, a component of cigarette smoke. It was performed in 2 different cell lines - GM12878 immortalized lymphoblast cell line and A549 lung cancer cell line.
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| Overall design |
We performed RNA-Seq in untreated GM12878 and A549 cells, as well as 6h after being treated with 5μM of BPDE, 200μM of cisplatin, 20J/m2 of UVC. For each condition there were 3 replicates: A549 control, A549 + cisplatin, A549 + BPDE , A549 + UV , GM12878 control, GM12878 + cisplatin, GM12878 + BPDE , GM12878 + UV.
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| Contributor(s) |
Merav M, Bitensky EM, Heilbrun EM, Hacohen T, Kirshenbaum A, Golan-Berman H, Cohen Y, Adar S |
| Citation(s) |
38167611 |
| |
| Submission date |
Jun 23, 2023 |
| Last update date |
Jan 16, 2024 |
| Contact name |
Sheera Adar |
| Organization name |
Hebrew University of Jerusalem
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| Department |
Department of Microbiology and Molecular Genetics
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| Lab |
Sheera Adar
|
| Street address |
Botnar Building Room 001, Faculty of Medicine, Hebrew University, Ein Kerem
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| City |
Jerusalem |
| State/province |
Israel |
| ZIP/Postal code |
91120 |
| Country |
Israel |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA986894 |
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