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| Status |
Public on Oct 27, 2023 |
| Title |
Fungal metabolism and free amino acid content may predict nitrogen transfer to the host plant in the ectomycorrhizal relationship between Pisolithus spp. and Eucalyptus grandis |
| Organisms |
Eucalyptus grandis; Pisolithus microcarpus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ectomycorrhizal (ECM) fungi are crucial for tree nitrogen (N) nutrition, however, mechanisms governing N transfer from fungal tissues to the host plant are not well understood. ECM fungal isolates, even from the same species, vary considerably in their ability to support tree N nutrition resulting in a range of often unpredictable symbiotic outcomes. In this study, we used isotopic labelling to quantify the transfer of N to the plant host by isolates from the ECM genus Pisolithus known to have significant variability in colonisation and transfer of nutrients to a host. We considered the metabolic fate of N acquired by the fungi and found that the percentage of plant N acquired through symbiosis significantly correlated to the concentration of free amino acids present in the ECM extra-radical mycelium. Transcriptomic analyses complemented these findings with isolates having high amino acid content and N transfer showing increased expression of genes in amino acid transport and catabolic pathways. These results suggest that fungal N metabolism drives transfer to the host plant in this interaction and that relative N transfer may be possible to predict through basic biochemical analyses.
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| Overall design |
In total 43 samples were analysed by RNA-sequencing of which 22 samples included 3-4 biological replicates each of 1 timepoint of symbiosis between Eucalyptus grandis and Pisolithus microcarpus (2 week post contact). The remaining samples included biological replicates of six genotypes of P. microcarpus grown axenically as a control and three biological replicates of axenically grown E. grandis. All organisms and symbiotic compartment was performed on ½ strength Modified Melin Norkans Medium (with 0.1g/L of glucose).
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| Web link |
https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.19400
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| Contributor(s) |
Plett JM, Plett KL, Anderson IC |
| Citation(s) |
37974494 |
| Submission date |
Jun 23, 2023 |
| Last update date |
Jan 29, 2024 |
| Contact name |
Jonathan Michael Plett |
| E-mail(s) |
j.plett@westernsydney.edu.au
|
| Organization name |
Western Sydney University
|
| Department |
Hawkesbury Institute for the Environment
|
| Street address |
Bourke St. Entrance, Building L9
|
| City |
Richmond |
| State/province |
NSW |
| ZIP/Postal code |
2753 |
| Country |
Australia |
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| Platforms (3) |
| GPL33522 |
Illumina NovaSeq 6000 (Pisolithus microcarpus) |
| GPL33523 |
Illumina NovaSeq 6000 (Eucalyptus grandis; Pisolithus microcarpus) |
| GPL33524 |
Illumina NovaSeq 6000 (Eucalyptus grandis) |
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| Samples (43)
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| Relations |
| BioProject |
PRJNA986873 |
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