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| Status |
Public on Sep 14, 2023 |
| Title |
ACD15, ACD21 and SLN regulate accumulation and mobility of MBD6 to silence genes and transposable elements [ChIP-seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
DNA methylation mediates silencing of transposable elements and genes in part via recruitment of the Arabidopsis MBD5/6 complex, which contains the methyl-CpG-binding domain (MBD) proteins MBD5 and MBD6, and the J-domain containing protein SILENZIO (SLN). Here we characterize two additional complex members: α-crystalline domain containing proteins ACD15 and ACD21. We show that they are necessary for gene silencing, bridge SLN to the complex, and promote higher order multimerization of MBD5/6 complexes within heterochromatin. These complexes are also highly dynamic, with the mobility of complex components regulated by the activity of SLN. Using a dCas9 system, we demonstrate that tethering the ACDs to an ectopic site outside of heterochromatin can drive massive accumulation of MBD5/6 complexes into large nuclear bodies. These results demonstrate that ACD15 and ACD21 are critical components of gene silencing complexes that act to drive the formation of higher order, dynamic assemblies.
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| Overall design |
This submission includes 11 ChIP-seq libraries.
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| Contributor(s) |
Boone BA, Ichino L, Wang S, Gardiner J, Yun J, Jami-Alahmadi Y, Sha J, Mendoza CP, Steelman BJ, van Aardenne A, Kira-Lucas S, Trentchev I, Wohlschlegel JA, Jacobsen SE |
| Citation(s) |
37662299, 37967186 |
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| Submission date |
Jun 08, 2023 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Lucia Ichino |
| E-mail(s) |
lucia.ichino@gmail.com
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| Organization name |
University of California, Los Angeles
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| Department |
MCDB
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| Lab |
Jacobsen
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| Street address |
610 Charles E Young Dr East
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE234532 |
ACD15, ACD21 and SLN regulate accumulation and mobility of MBD6 to silence genes and transposable elements |
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| Relations |
| BioProject |
PRJNA981638 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE234531_ACD15_merged_peaks_edit_minusCol0.txt.gz |
241.0 Kb |
(ftp)(http) |
TXT |
| GSE234531_ACD21_merged_peaks_edit_minusCol0.txt.gz |
143.7 Kb |
(ftp)(http) |
TXT |
| GSE234531_RAW.tar |
375.1 Mb |
(http)(custom) |
TAR (of BED, BW, TXT) |
| GSE234531_merge_broad_peaksMBD56_edit.txt.gz |
99.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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