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| Status |
Public on Dec 20, 2023 |
| Title |
Gene expression profile at single cell level of murine B cells from different compartments of naive young adult mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
In the context of understanding thymic T cell selection facilitated by local B cells, we sought to compare thymic B cells with their counterparts from other compartments in naive young adult mice.
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| Overall design |
B cells from spleen, inguinal lymph nodes, bone marrow, thymus and blood (each pooled from two mice) were isolated by fluorescence-activated cell sorting (FACS) and analyzed using scRNAseq (10x).
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| Contributor(s) |
Korn T, Afzali A, Nirschl L, Sie C |
| Citation(s) |
38383779 |
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| Submission date |
Jun 05, 2023 |
| Last update date |
Feb 22, 2024 |
| Contact name |
Thomas Korn |
| E-mail(s) |
thomas.korn@tum.de, c.sie@tum.de
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| Organization name |
Technical University of Munich
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| Department |
Experimental Neuroimmunology
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| Street address |
Ismaninger Str. 22
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| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (10)
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| GSM7453694 |
B cells from spleen, scRNA-seq, GEX |
| GSM7453695 |
B cells from spleen, scRNA-seq, HTO |
| GSM7453696 |
B cells from inguinal lymph nodes, scRNA-seq, GEX |
| GSM7453697 |
B cells from inguinal lymph nodes, scRNA-seq, HTO |
| GSM7453698 |
B cells from bone marrow, scRNA-seq, GEX |
| GSM7453699 |
B cells from bone marrow, scRNA-seq, HTO |
| GSM7453700 |
B cells from thymus, scRNA-seq, GEX |
| GSM7453701 |
B cells from thymus, scRNA-seq, HTO |
| GSM7453702 |
B cells from blood, scRNA-seq, GEX |
| GSM7453703 |
B cells from blood, scRNA-seq, HTO |
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| Relations |
| BioProject |
PRJNA980254 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE234188_Sample_01_SPL_Velocyto.loom.gz |
39.8 Mb |
(ftp)(http) |
LOOM |
| GSE234188_Sample_01_SPL_barcodes.tsv.gz |
26.4 Kb |
(ftp)(http) |
TSV |
| GSE234188_Sample_01_SPL_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE234188_Sample_01_SPL_matrix.mtx.gz |
20.5 Mb |
(ftp)(http) |
MTX |
| GSE234188_Sample_02_LN_Velocyto.loom.gz |
28.3 Mb |
(ftp)(http) |
LOOM |
| GSE234188_Sample_02_LN_barcodes.tsv.gz |
18.9 Kb |
(ftp)(http) |
TSV |
| GSE234188_Sample_02_LN_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE234188_Sample_02_LN_matrix.mtx.gz |
15.2 Mb |
(ftp)(http) |
MTX |
| GSE234188_Sample_03_BM_Velocyto.loom.gz |
34.5 Mb |
(ftp)(http) |
LOOM |
| GSE234188_Sample_03_BM_barcodes.tsv.gz |
20.1 Kb |
(ftp)(http) |
TSV |
| GSE234188_Sample_03_BM_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE234188_Sample_03_BM_matrix.mtx.gz |
18.2 Mb |
(ftp)(http) |
MTX |
| GSE234188_Sample_04_THY_Velocyto.loom.gz |
38.7 Mb |
(ftp)(http) |
LOOM |
| GSE234188_Sample_04_THY_barcodes.tsv.gz |
23.3 Kb |
(ftp)(http) |
TSV |
| GSE234188_Sample_04_THY_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE234188_Sample_04_THY_matrix.mtx.gz |
19.3 Mb |
(ftp)(http) |
MTX |
| GSE234188_Sample_05_Blood_Velocyto.loom.gz |
29.2 Mb |
(ftp)(http) |
LOOM |
| GSE234188_Sample_05_Blood_barcodes.tsv.gz |
18.9 Kb |
(ftp)(http) |
TSV |
| GSE234188_Sample_05_Blood_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE234188_Sample_05_Blood_matrix.mtx.gz |
15.7 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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