 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 20, 2024 |
| Title |
H3K4 methylation regulates development, DNA repair, and virulence in Mucorales |
| Organism |
Mucor lusitanicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mucorales are basal fungi that opportunistically cause a fatal infection known as mucormycosis (black fungus disease), which poses a significant threat to human health due to its high mortality rate and its recent association with SARS-CoV-2 infections. On the other hand, histone methylation is a regulatory mechanism with pleiotropic effects, including the virulence of several pathogenic organisms. However, the role of epigenetic changes at the histone level never has been studied in Mucorales. Here, we dissected the functional role of Set1, a histone methyltransferase that catalyzes the methylation of H3K4, which is associated with the activation of gene transcription and virulence. A comparative analysis of the Mucor lusitanicus genome (previously known as Mucor circinelloides f. lusitanicus) identified only one homolog of Set1 from Candida albicans and Saccharomyces cerevisiae that contains the typical SET domain. Knockout strains in the gene set1 lacked H3K4 monomethylation, dimethylation, and trimethylation enzymatic activities. These strains also showed a significant reduction in vegetative growth and sporulation. Additionally, set1 null strains were more sensitive to SDS, EMS, and UV light, indicating severe impairment in the repair process of the cell wall and DNA lesions and a correlation between Set1 and these processes. During pathogen-host interactions, strains lacking the set1 gene exhibited shortened polar growth within the phagosome and attenuated virulence both in vitro and in vivo. Our findings suggest that the histone methyltransferase Set1 coordinates several cell processes related to the pathogenesis of M. lusitanicus and may be an important target for future therapeutic strategies against mucormycosis.
|
| |
|
| Overall design |
RNA-seq experiment to analyze the differences in gene expression caused by Set1 KO in M. lusitanicus. Total RNA was extracted from MU636 and MU1350 (set1 KO) for 24 hours on YPG media (solid) at 26 ºC. For each strain, we performed three biological replicates
|
| |
|
| Contributor(s) |
Lax C, Cánovas-Márquez JT |
| Citation(s) |
38481304 |
| |
| Submission date |
Jun 01, 2023 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Carlos Lax |
| E-mail(s) |
carlos.lax@um.es
|
| Organization name |
University of Murcia
|
| Department |
Genetics and Microbiology
|
| Street address |
Facultad de Biología
|
| City |
Murcia |
| ZIP/Postal code |
30100 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL33451 |
Illumina NovaSeq 6000 (Mucor lusitanicus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA978531 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE233894_Raw_counts_set.xlsx |
411.3 Kb |
(ftp)(http) |
XLSX |
| GSE233894_S2_Dataset.xlsx |
65.5 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...