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| Status |
Public on Feb 07, 2011 |
| Title |
Human SV80 Cells: Control vs. Caspase-8 knock down |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Excessive responses to pattern-recognition receptors are prevented by regulatory mechanisms that affect the amounts, conformation, and associative properties of their signaling proteins. We report that signaling by the ribonucleic acid sensor RIG-I is restricted, in addition, by caspase-mediated cleavage that results in conversion of a signaling enhancer to a signaling inhibitor. RIP1 and caspase-8, two proteins known to mediate effects of receptors of the TNF/NGF family, are recruited to the RIG-I complex following viral infection, and serve in a coordinated manner antagonistic regulatory roles: conjugation of a ubiquitin chain to Lys-377 in RIP1 facilitates assembly of the RIG-I complex, but it also renders RIP1 susceptible to caspase-8-mediated cleavage, yielding an inhibitory RIP1 fragment. The dependence of RIP1 cleavage on the same molecular change as the one that facilitates RIG-I signaling allows for RIG-I signaling to be restricted in its duration without compromising its initial activation.
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| Overall design |
Transcriptional profiling of human SV80 cells comparing cells infected with control lenti virus to cells infected with caspase-8 siRNA expressing lenti virus. Goal was to determine the effects of caspase-8 knock down on global gene expression.
Two-condition experiment, Control lenti Vs caspase-8 siRNA expressing lenti. Biological replicates: 4 control, 4 caspase-8 siRNA infected
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| Contributor(s) |
Wallach D, Rajput A |
| Citation(s) |
21419663 |
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| Submission date |
Aug 02, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Akhil Rajput |
| E-mail(s) |
akhil.rajput@weizmann.ac.il
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| Organization name |
The Weizmann Institute
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| Department |
Biological Chemistry
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| Street address |
1, Herzl
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| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA131251 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23363_RAW.tar |
63.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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