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| Status |
Public on Jun 02, 2023 |
| Title |
Effect of depletion of Notch signaling genes in C2C12 cells on the activation of Notch pathway activity in response to stimulation by plated ligand |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To obtain a Notch-depleted base cell line into which we could integrate specific ligands or receptors for quantitative analysis of Notch signaling interactions,
we conducted 2 rounds of transfection with RNPs (ribonucleoproteins) consisting of gRNAs targeting Notch2 and Jagged1 complexed with Cas9 protein.
After antibiotic selection and screening of monoclonal colonies, we obtained the C2C12-Nkd clone, which showed loss of Notch2 protein and Jag1 transcript.
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| Overall design |
Here, we analyze the transcriptome of this C2C12-Nkd line compared with wild-type C2C12, and its response to stimulation of Notch signaling by plated recombinant ligands.
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| Contributor(s) |
Rachael K, Leah S, Michael E |
| Citation(s) |
37662208 |
| |
| Submission date |
May 26, 2023 |
| Last update date |
Sep 15, 2023 |
| Contact name |
Rachael Kuintzle |
| E-mail(s) |
rachaelkuintzle@gmail.com
|
| Phone |
5305186895
|
| Organization name |
California Institute Of Technology
|
| Department |
Chemistry and Chemical Engineering
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| Lab |
Elowitz Lab
|
| Street address |
391 S Holliston Ave, Bio 73, MC 140-18
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| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA976680 |
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