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| Status |
Public on Aug 26, 2011 |
| Title |
Transcriptome analysis of vtc2, abi4-102 and the corresponding double mutant abi4 vtc2 |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The role of abscisic acid (ABA) signalling in the ascorbic acid (AA)-dependent control of plant growth and defence was determined using the vtc1 and vtc2 mutants, which have impaired ascorbic acid synthesis, and in the abi4 mutant that is impaired in ABA-signalling. ABA levels were increase in the mutants relative to the wild type (Col0). Like vtc1 the vtc2 mutants have a slow growth relative to Col0. However, the wild type phenotype is restored in the abi4vtc2 double mutant. Similarly, the sugar sensing phenotype of in the abi4 is reversed in the abi4vtc2 double mutant. The vtc1 and vtc2 leaf transcriptomes show up to 70 % homology with abi4. Of the transcripts that are altered in the mutants a relative to Col0, only a small number are reversed in the abi4vtc2 double mutants relative to either abi4 or vtc2. We conclude that AA controls growth via an ABA and abi4-dependent signalling pathway. The vtc and abi4 mutants have enhanced glutathione levels and common redox signalling pathways leading to similar gene expression patterns.
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| Overall design |
Rosettes of 42 days old plants were harvested and used to exctract RNA
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| Contributor(s) |
Kerchev P, Foyer CH |
| Citation(s) |
21926335 |
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| Submission date |
Jul 29, 2010 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Pavel Kerchev |
| E-mail(s) |
pavel.kerchev@scri.ac.uk
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| Organization name |
University of Leeds
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| Street address |
Faculty of Biological Sciences University of Leeds
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| City |
Leeds |
| ZIP/Postal code |
LS2 9JT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (17)
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| GSM572630 |
Rosette of Col 0, biological rep1 (I) |
| GSM572631 |
Rosette of Col 0, biological rep2 (I) |
| GSM572632 |
Rosette of Col 0, biological rep3 (I) |
| GSM572633 |
Rosette of abi4-102, biological rep1 (I) |
| GSM572634 |
Rosette of abi4-102, biological rep2 (I) |
| GSM572635 |
Rosette of abi4-102, biological rep3 (I) |
| GSM572636 |
Rosette of abi4-102, biological rep4 (I) |
| GSM572637 |
Rosette of vtc2, biological rep1 (I) |
| GSM572638 |
Rosette of vtc2, biological rep2 (I) |
| GSM572639 |
Rosette of vtc2, biological rep3 (I) |
| GSM572640 |
Rosette of vtc2, biological rep4 (I) |
| GSM572641 |
Rosette of abi4 vtc2, biological rep1 (I) |
| GSM572642 |
Rosette of abi4 vtc2, biological rep2 (I) |
| GSM572643 |
Rosette of abi4 vtc2, biological rep3 (I) |
| GSM572644 |
Rosette of abi4 vtc2, biological rep4 (I) |
| GSM572645 |
Rosette of abi4 vtc2, biological rep5 (I) |
| GSM572646 |
Rosette of abi4 vtc2, biological rep6 (I) |
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| This SubSeries is part of SuperSeries: |
| GSE23331 |
Ascorbic acid-dependent regulation of growth involves abscisic acid signalling through ABI-4 |
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| Relations |
| BioProject |
PRJNA133295 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23329_RAW.tar |
31.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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