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| Status |
Public on May 15, 2023 |
| Title |
Lin28 is essential for the maintenance of chicken primordial germ cells |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the function of Lin28A and B in primordial germ cell (PGC) , we generated chicken PGCs in which Lin28A or B has been knocked out by CRISPR/Cas9.
We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different cell types.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for (1) control, (2) Lin28A knockout, (3) Lin28B knockout chicken PGCs.
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| Contributor(s) |
Kwon SJ, Suzuki K, Atsuta Y |
| Citation(s) |
37453484 |
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| Submission date |
May 09, 2023 |
| Last update date |
Aug 16, 2023 |
| Contact name |
Seung June Kwon |
| E-mail(s) |
udnjf251@gmail.com
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| Phone |
+81-90-4761-6419
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| Organization name |
Kyushu University
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| Department |
Graduate School of Systems Life Sciences
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| Lab |
Laboratory of Animal Developmental Biology
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| Street address |
744 Motooka, Nishi-ku
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| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
819-0395 |
| Country |
Japan |
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| Platforms (1) |
| GPL26853 |
Illumina NovaSeq 6000 (Gallus gallus) |
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| Samples (9)
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| GSM7310620 |
Primordial germ cells, Lin28-aKO, rep1 |
| GSM7310621 |
Primordial germ cells, Lin28-aKO, rep2 |
| GSM7310622 |
Primordial germ cells, Lin28-aKO, rep3 |
| GSM7310623 |
Primordial germ cells, Lin28-bKO, rep1 |
| GSM7310624 |
Primordial germ cells, Lin28-bKO, rep2 |
| GSM7310625 |
Primordial germ cells, Lin28-bKO, rep3 |
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| Relations |
| BioProject |
PRJNA970677 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE232032_DESeqResult_LIN28AKO_Ctrl.xlsx |
1.5 Mb |
(ftp)(http) |
XLSX |
| GSE232032_DESeqResult_LIN28BKO_Ctrl.xlsx |
1.5 Mb |
(ftp)(http) |
XLSX |
| GSE232032_RAW.tar |
1.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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