|
| Status |
Public on May 12, 2023 |
| Title |
Genome-wide analysis of gene expression by B. subtilis biofilms grown in buffered or minimally buffered conditions, |
| Organism |
Bacillus subtilis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Analysis of B. subtilis biofilm metabolism at gene expression level. The hypothesis tested in the present study was that extracellular pH and environmental buffer influence the biofilm development. Results provide important information of the response of biofilms to changes in extracellular pH.
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| |
|
| Overall design |
Total RNA obtained from B. subtilis NCIB 3610 WT or ∆alsS mutant biofoilms grown on MSgg with 1 mM MOPS (minimally buffered) at 24 or 36 h compared to NCIB 3610 WT or ∆alsS mutant biofoilms grown on MSgg with 100 mM MOPS (buffered) at 24 or 36 h
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| |
|
| Contributor(s) |
Tran P, Prindle A |
| Citation(s) |
38349175 |
| |
| Submission date |
May 08, 2023 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Peter Tran |
| E-mail(s) |
ptran@u.northwestern.edu
|
| Phone |
8133913337
|
| Organization name |
Prindle Lab, Northwestern University
|
| Street address |
impson Querrey Research Bldg 302 E Huron St, Room 11-500
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL29515 |
Illumina HiSeq 4000 (Bacillus subtilis) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA970277 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE231939_24h_WT_control_vs_36h_WT_control_diff_expression_with_norm.xlsx |
2.6 Mb |
(ftp)(http) |
XLSX |
| GSE231939_36h_alsS_control_vs_36_h_alsS_UB_diff_expression_with_norm.xlsx |
2.7 Mb |
(ftp)(http) |
XLSX |
| GSE231939_S24h_WT_UB_vs_S24h_alsS_UB_deseq_diff_expression_with_norm.xlsx |
2.6 Mb |
(ftp)(http) |
XLSX |
| GSE231939_S24h_WT_UB_vs_S36h_WT_UB_deseq_diff_expression_with_norm.xlsx |
2.6 Mb |
(ftp)(http) |
XLSX |
| GSE231939_S24h_WT_control_vs_S24h_WT_UB_deseq_diff_expression_with_norm.xlsx |
2.6 Mb |
(ftp)(http) |
XLSX |
| GSE231939_S24h_WT_control_vs_S24h_alsS_control.xlsx |
814.2 Kb |
(ftp)(http) |
XLSX |
| GSE231939_S24h_alsS_UB_vs_S36_h_alsS_UB_deseq_diff_expression_with_norm.xlsx |
2.7 Mb |
(ftp)(http) |
XLSX |
| GSE231939_S24h_alsS_control_vs_S24h_alsS_UB_deseq_diff_expression_with_norm.xlsx |
2.6 Mb |
(ftp)(http) |
XLSX |
| GSE231939_S36h_WT_UB_vs_S36_h_alsS_UB_deseq_diff_expression_with_norm.xlsx |
2.8 Mb |
(ftp)(http) |
XLSX |
| GSE231939_S36h_WT_control_vs_S36h_WT_UB_deseq_diff_expression_with_norm.xlsx |
2.7 Mb |
(ftp)(http) |
XLSX |
| GSE231939_S36h_WT_control_vs_S36h_alsS_control.xlsx |
827.2 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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