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| Status |
Public on May 01, 2023 |
| Title |
A core of differentially methylated CpG loci in gMDSCs isolated from neonatal and adult sources |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
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| Summary |
Using the Illumina EPIC array to measure DNAm of gMDSCs and neutrophils from diverse neonatal and adult blood sources, we found 189 differentially methylated CpGs between gMDSCs and neutrophils with a core of ten differentially methylated CpGs that were consistent across both sources of cells. Genes associated with these loci that are involved in immune responses include VCL, FATS, YAP1, KREMEN2, UBTF, MCC-1, and EFCC1. In two cancer patient groups that reflected those used to develop the methylation markers (head and neck squamous cell carcinoma (HNSCC) and glioma), all of the CpG loci were differentially methylated, reaching statistical significance in glioma cases and controls, while one was significantly different in the smaller HNSCC group. Our findings indicate that gMDSCs have a core of distinct DNAm alterations, informing future research on gMDSC differentiation and function.
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| Overall design |
Bisulphite converted DNA from fourteen gMDSC cells were hybridised to the Illumina Infinium HumanMethylationEPIC Beadchip v1.0_B4
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| Contributor(s) |
Berglund-Brown I, Nissen E, Koestler DC, Butler RA, Eliot MN, Padbury JF, Salas LA, Molinaro AM, Christensen BC, Wiencke JK, Kelsey KT, Zhang Z |
| Citation(s) |
35189960 |
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| Submission date |
May 01, 2023 |
| Last update date |
May 04, 2023 |
| Contact name |
Ze Zhang |
| E-mail(s) |
ze_zhang@dfci.harvard.edu
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| Organization name |
Harvard Medical School
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| Department |
Medical Oncology & Population Sciences
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| Lab |
Baca Lab
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| Street address |
375 Longwood Ave
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL23976 |
Illumina Infinium HumanMethylation850 BeadChip |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA964938 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE231417_Matrix_signal_intensities.txt.gz |
200.9 Mb |
(ftp)(http) |
TXT |
| GSE231417_RAW.tar |
196.4 Mb |
(http)(custom) |
TAR (of IDAT) |
| Processed data included within Sample table |
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