|
| Status |
Public on Feb 21, 2024 |
| Title |
Single-cell RNA-sequencing and single-cell TCR-sequencing of tumour-infiltrating CD8+ T cells derived from mouse BRAFV600E melanoma [scRNA-Seq + TCR 10x] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single-cell RNA-sequencing and single-cell TCR-sequencing was used to characterize PGE2-mediated changes in the gene expression profile of tumor-infiltrating CD8+ T cells.
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|
| Overall design |
Ptger2-/-Ptger4fl/fl, CD4CrePtger2-/-Ptger4fl/fl, GzmBCrePtger2-/-Ptger4fl/fl and C57BL/6 wildtype mice were subcutaneously injected with BRAFV600E tumour cells at their flanks. Tumours were harvested on day 11 post tumour cell inoculation and tumour-infiltrating CD8+ T cells were sorted by FACS. Each experimental group consisted of 4 biological replicates.
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| |
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| Contributor(s) |
Böttcher J, Lacher S, de Almeida GP |
| Citation(s) |
38658748 |
| |
| Submission date |
Apr 29, 2023 |
| Last update date |
May 17, 2024 |
| Contact name |
Gustavo P. de Almeida |
| E-mail(s) |
gustavo.almeida@tum.de
|
| Organization name |
Technical University of Munich
|
| Department |
Division of Animal Physiology and Immunology
|
| Street address |
Liesel-Beckmann-Str. 1
|
| City |
Freising |
| State/province |
Bavaria |
| ZIP/Postal code |
85354 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (12)
|
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| This SubSeries is part of SuperSeries: |
| GSE231340 |
PGE2 curtails IL-2-dependent effector expansion from tumour-infiltrating stem-like CD8+ T cells to promote cancer immune escape |
|
| Relations |
| BioProject |
PRJNA963168 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE231302_C57BL6_BRAFV600E_1.tar.gz |
11.7 Mb |
(ftp)(http) |
TAR |
| GSE231302_C57BL6_BRAFV600E_2.tar.gz |
13.8 Mb |
(ftp)(http) |
TAR |
| GSE231302_C57BL6_BRAFV600E_3.tar.gz |
22.1 Mb |
(ftp)(http) |
TAR |
| GSE231302_C57BL6_BRAFV600E_4.tar.gz |
32.3 Mb |
(ftp)(http) |
TAR |
| GSE231302_CD4_Cre_Ptger2dd_Ptger4ff_BRAFV600E_1.tar.gz |
33.5 Mb |
(ftp)(http) |
TAR |
| GSE231302_CD4_Cre_Ptger2dd_Ptger4ff_BRAFV600E_2.tar.gz |
32.2 Mb |
(ftp)(http) |
TAR |
| GSE231302_CD4_Cre_Ptger2dd_Ptger4ff_BRAFV600E_3.tar.gz |
28.4 Mb |
(ftp)(http) |
TAR |
| GSE231302_CD4_Cre_Ptger2dd_Ptger4ff_BRAFV600E_4.tar.gz |
30.7 Mb |
(ftp)(http) |
TAR |
| GSE231302_GzmB_Cre_Ptger2dd_Ptger4ff_BRAFV600E_1.tar.gz |
37.6 Mb |
(ftp)(http) |
TAR |
| GSE231302_GzmB_Cre_Ptger2dd_Ptger4ff_BRAFV600E_2.tar.gz |
32.7 Mb |
(ftp)(http) |
TAR |
| GSE231302_GzmB_Cre_Ptger2dd_Ptger4ff_BRAFV600E_3.tar.gz |
25.6 Mb |
(ftp)(http) |
TAR |
| GSE231302_GzmB_Cre_Ptger2dd_Ptger4ff_BRAFV600E_4.tar.gz |
30.3 Mb |
(ftp)(http) |
TAR |
| GSE231302_Ptger2dd_Ptger4ff_BRAFV600E_1.tar.gz |
17.3 Mb |
(ftp)(http) |
TAR |
| GSE231302_Ptger2dd_Ptger4ff_BRAFV600E_2.tar.gz |
28.5 Mb |
(ftp)(http) |
TAR |
| GSE231302_Ptger2dd_Ptger4ff_BRAFV600E_3.tar.gz |
24.3 Mb |
(ftp)(http) |
TAR |
| GSE231302_Ptger2dd_Ptger4ff_BRAFV600E_4.tar.gz |
22.2 Mb |
(ftp)(http) |
TAR |
| GSE231302_tumor_CD8T_cells_metadata_norm.txt.gz |
45.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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