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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 02, 2023 |
| Title |
Understanding the cell fate and behavior of progenitors at the origin of the mouse cardiac mitral valve |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Congenital heart malformations include mitral valve defects, which remain largely unexplained. During embryogenesis, a restricted population of endocardial cells within the atrioventricular canal undergoes an endothelial-to-mesenchymal transition to give rise to mitral valvular cells. However, the identity and fate decisions of these progenitors as well as the behavior and distribution of their derivatives in valve leaflets remain unknown. We used single-cell RNA sequencing (scRNA-seq) of genetically labeled endocardial cells and microdissected mouse embryonic and postnatal mitral valves to characterize the developmental road. We defined the metabolic processes underlying the specification of the progenitors and their contributions to subtypes of valvular cells. Using retrospective multicolor clonal analysis, we describe specific modes of growth and behavior of endocardial cell-derived clones, which build up, in a proper manner, functional valve leaflets. Our data identify how both genetic and metabolic mechanisms specifically drive the fate of a subset of endocardial cells toward their distinct clonal contribution to the formation of the valve.
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| Overall design |
Cells were isolated from enbryos from Tie2crex Rosa26 tdTomato female mice after microdissection of the AVC and embryonic mitral valves. At least 60 embryos were used for each stage of development Cells were then FACS sorted using tomato as a reporter and used in 10X chromium
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| Contributor(s) |
Farhat B, Bordeu I, Jagla B, Blanc H, Loulier K, Simons BD, Beaurepaire E, Livet J, Pucéat M |
| Citation(s) |
38198889 |
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| Submission date |
Apr 28, 2023 |
| Last update date |
Mar 03, 2024 |
| Contact name |
michel PUCEAT |
| E-mail(s) |
michel.puceat@inserm.fr
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| Phone |
33 4 91 32 49 47
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| Organization name |
INSERM
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| Department |
University aix marseille
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| Lab |
physiopathology of cardiac development
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| Street address |
27 bd Jean moulin
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| City |
Marseille |
| ZIP/Postal code |
13885 |
| Country |
France |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA962839 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE230849_RAW.tar |
25.1 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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