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| Status |
Public on Jul 20, 2011 |
| Title |
Gene expression profiling of HUVEC under basal (resting) conditions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To understand the biological variability that exists between different individuals, the resting (untreated) transcriptome from 15 different individuals was examined. Transcripts expressed in a bimodal manor across the 15 biological replicates were identified for further examination.
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| Overall design |
In the study presented here, HUVEC isolates from 15 different individuals were cultured until passage 4 in fully supplemented growth conditions. RNA was extracted and hybridised onto CodeLink microarrays. The expression profiles in the untreated condition were examined along side a subset of the same biological replicates treated with a cocktail of inflammatory mediators (10ng/ml TNF-α, Il-1β, Il-8) for a period of 24 hours prior to RNA extraction (also submitted).
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| Contributor(s) |
Affara M, Charnock-Jones DS, Print C |
| Citation(s) |
21244711 |
| |
| Submission date |
Jul 21, 2010 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Muna Affara |
| E-mail(s) |
muna.affara@gmail.com
|
| Organization name |
University of Cambridge
|
| Department |
Pathology
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1QP |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL10716 |
Cambridge/CodeLink UniSet Human 20K I Bioarray |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE23070 |
*MMP1* bimodal expression and differential response to inflammatory mediators is linked to promoter polymorphisms |
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| Relations |
| BioProject |
PRJNA133105 |
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