|
| Status |
Public on Oct 13, 2023 |
| Title |
Small molecule-mediated reprogramming of Xenopus blastula stem cells to a neural crest state |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here we describe and validate a protocol for small molecule-mediated induction of the neural crest state
as a new tool for generating this important cell type and facilitating future studies of its development.
|
| |
|
| Overall design |
We induce a neural crest state using a BMP inhibitor (K02288) and wnt agonist (CHIR99021) and compare
the transcriptome to other neural crest data sets.
|
| Web link |
https://pubmed.ncbi.nlm.nih.gov/37890713/
|
| |
|
| Contributor(s) |
Huber P, LaBonne C |
| Citation(s) |
37890713 |
| |
| Submission date |
Apr 22, 2023 |
| Last update date |
Jan 12, 2024 |
| Contact name |
Paul Benjamin Huber |
| E-mail(s) |
paul.huber@northwestern.edu
|
| Organization name |
Northwestern University
|
| Department |
Molecular Biosciences
|
| Lab |
LaBonne
|
| Street address |
2205 Tech Drive
|
| City |
Evanston |
| State/province |
Illinois |
| ZIP/Postal code |
60208 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18936 |
Illumina HiSeq 2500 (Xenopus laevis) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA960645 |
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