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| Status |
Public on Jun 27, 2024 |
| Title |
Effect of L. infantum infection in microtranscriptome of human neutrophils in vitro [Expression] |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
To understand the expression patterns of miRNAs expression during acute in vitro infection of human neutrophils by L. infantum we performed a microarray.
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| Overall design |
We isolated neutrophils from whole blood of healthy male donors; cells from each subject (n=5) were split into groups: 1) infected with L. infantum (MOI = 5:1), and 2) uninfected controls. After 3 hours of exposure of infected group to promastigotes of L. infantum, followed by 17 hours of incubation, total RNA was extracted miRNA microarray were performed.
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| Contributor(s) |
Scaramele NF, Troiano JA, Felix JS, Costa SF, Almeida MC, Athayde FF, Soares MF, Lopes MS, Furlan AO, Lima VM, Lopes FL |
| Citation(s) |
39028711 |
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| Submission date |
Apr 18, 2023 |
| Last update date |
Jul 21, 2024 |
| Contact name |
Flavia Lombardi Lopes |
| E-mail(s) |
flavia.lopes@unesp.br
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| Phone |
551836360032
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| Organization name |
FMVA-UNESP
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| Department |
DAPSA
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| Lab |
Epigenomics Lab
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| Street address |
Rua Clovis Pestana, 793
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| City |
Araçatuba |
| State/province |
São Paulo |
| ZIP/Postal code |
16050-680 |
| Country |
Brazil |
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| Platforms (1) |
| GPL21572 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version] |
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| Samples (10)
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| GSM7183956 |
neutrophils, uninfected, subject 07 |
| GSM7183957 |
neutrophils, uninfected, subject 08 |
| GSM7183958 |
neutrophils, infected with L. infantum, subject 02 |
| GSM7183959 |
neutrophils, infected with L. infantum, subject 05 |
| GSM7183960 |
neutrophils, infected with L. infantum, subject 06 |
| GSM7183961 |
neutrophils, infected with L. infantum, subject 07 |
| GSM7183962 |
neutrophils, infected with L. infantum, subject 08 |
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| Relations |
| BioProject |
PRJNA956968 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE230017_RAW.tar |
8.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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