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Series GSE2299 Query DataSets for GSE2299
Status Public on Dec 01, 2005
Title Egr-1 induced gene expression changes in HUVECs
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The target genes of the transcription factor early growth response-1 (EGR-1) were studied in human umbilical vein endothelial cells (HUVEC). Densely cultured HUVEC were infected with recombinant adenoviruses expressing EGR-1 or empty control viruses at a MOI of 100. RNA was isolated from the cells at the time points 16 h, 24 h and 48 h post infection. Control cells without infection were cultured in parallel and harvested at the 24 h time point. Total RNA was isolated using TRIzol (InVitrogen) and Alater (Ambion) reagents and cDNA synthesis, hybridization and microarray analysis using U133 GeneChips performed according to the Affymetrix manual. Gene expression changes were calculated as the ratio of EGR-1 or control virus (CV) infected cells to non-infected control cells.
Keywords: time-course
Contributor(s) Pomyje J, Bilban M, Hofer E
Citation(s) 16818645
Submission date Feb 19, 2005
Last update date Aug 10, 2018
Contact name Martin Bilban
Phone ++43 (0)1 40400 6441
Organization name Medical University of Vienna
Street address
City Vienna
ZIP/Postal code 1090
Country Austria
Platforms (2)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL97 [HG-U133B] Affymetrix Human Genome U133B Array
Samples (14)
GSM42864 egr1 16 hrs U133A
GSM42865 egr1 16 hrs cv U133A
GSM42866 egr1 24 hrs U133A
BioProject PRJNA91679

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