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| Status |
Public on Sep 01, 2010 |
| Title |
The Nuclear-retained non-coding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Alternative splicing (AS) of pre-mRNA is utilized by higher eukaryotes to achieve increased transcriptome and proteomic complexity. The serine/arginine (SR) splicing factors regulate tissue- or cell type-specific AS in a concentration and phosphorylation dependent manner. However, the mechanisms that modulate the cellular levels of active SR proteins remain to be elucidated. In the present study, we provide evidence for a role for the long nuclear-retained regulatory RNA (nrRNA), MALAT1 in AS regulation. MALAT1 interacts with SR proteins and influences the distribution of these and other splicing factors in nuclear speckle domains. Depletion of MALAT1 changes AS of endogenous pre-mRNAs, similar to what was observed upon overexpression of SR proteins. Furthermore, MALAT1 regulates cellular levels of phosphorylated forms of SR proteins. Taken together, our results suggest that MALAT1 regulates AS by modulating the levels of active SR proteins. Our results further highlight a novel role for a nrRNA in the regulation of gene expression.
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| Overall design |
Malat1 Antisense and control knockdowns evaluated on a microarray platform to profile alternative splicing levels for 5782 cassette-type alternative exons.
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| Contributor(s) |
Tripathi V, Ellis JD, Shen Z, Song DY, Pan Q, Watt AT, Freier SM, Frank Bennett C, Sharma A, Bubulya PA, Blencowe BJ, Prasanth SG, Prasanth KV |
| Citation(s) |
20797886 |
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| Submission date |
Jul 15, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Qun Pan |
| E-mail(s) |
qun.pan@utoronto.ca
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| Organization name |
University of Toronto
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| Street address |
160 College St.
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| City |
Toronto |
| ZIP/Postal code |
M5S 3E1 |
| Country |
Canada |
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| Platforms (1) |
| GPL10691 |
Agilent Human 244K Alternative Splicing Oligo Custom Microarray |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA127851 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22963_RAW.tar |
3.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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