 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 12, 2023 |
| Title |
Characterization of immature ovarian teratomas through single-cell transcriptome |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Immature ovarian teratomas are malignant germ cell tumor composed of complicated cell types and are characterized by pathological features of immature neuroectodermal tubules/rosettes. To reveal the heterogeneity, evolution trajectory and cell communications among tumor microenvironment, we performed single-cell RNA sequencing (scRNA-seq) on three patient-derived immature ovarian teratomas (PDT) samples, and conducted systematically comparison with stem cell lines derived immature teratomas (CDT). A total of qualified 22,153 cells were obtained and divided into 28 clusters, which can match to the scRNA-seq annotation of CDT as well as human fetal Cell Atlas, but with higher heterogeneity and more prolific cell-cell crosstalk. Radial glia cells (tagged by SOX2) and immature neuron (tagged by DCX) exhibited mutually exclusive expression, and differentiated along distinct evolutionary trajectory from cycling neural progenitors. Proportions of these neuroectodermal cell subtypes may play important roles in PDT through contributing to the internal heterogeneity of PDTs. Moreover, the immune cells in PDTs were infiltrated rather than teratoma-derived, with more abundant macrophage in immature neuron than those in radial glia cells, and the infiltrated macrophage subtypes (i.e., M1 and M2) were significantly correlated to clinical grade. Overall, suppressed evolution process and transcriptome regulation in neuroectodermal cells, reduced cell-cell crosstalk, higher M1/M2 proportion ratio, and enhanced T cell effects in tumor microenvironment are enriched in patients with favorable prognosis. In conclusion, we provided the comprehensive profile of PDT at single cell level and highlighted the potential usage of CDTs as a model for research on PDT.
|
| |
|
| Overall design |
scRNAseq data of three patient-derived immature ovarian teratomas (PDT) grade 3 samples
-------------------------------------
Authors state "it is prohibited to upload raw human data to foreign databases by Chinese laws".
|
| |
|
| Contributor(s) |
Cao M, Deng Y, Deng Y, Wu J, Yang C, Wang Z, Hou Q, Fu H, Ren Z, Xia X, Li Y, Wang W, Xu H, Liao X, Shu Y |
| Citation(s) |
36936909 |
| |
| Submission date |
Apr 10, 2023 |
| Last update date |
Apr 12, 2023 |
| Contact name |
Heng Xu |
| E-mail(s) |
xuheng81916@gmail.com
|
| Phone |
+86-15928058711
|
| Organization name |
Sichuan University
|
| Street address |
17#, section 3, South Renmin Road
|
| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (3) |
| GSM7159110 |
PDT01, Immature ovarian teratomas, case01 |
| GSM7159111 |
PDT02, Immature ovarian teratomas, case02 |
| GSM7159112 |
PDT03, Immature ovarian teratomas, case03 |
|
| Relations |
| BioProject |
PRJNA954033 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE229343_seurat_for_geo.Rds.gz |
2.0 Gb |
(ftp)(http) |
RDS |
| Raw data not provided for this record |
| Processed data are available on Series record |
|
|
|
|
 |
