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| Status |
Public on Dec 14, 2023 |
| Title |
Whole genome bisulfite-seq analysis of control and Dnmt3a conditional knockout (cKO) retinal ganglion cells at 2 days post optic nerve crush |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Dnmt3a deficiency in retinal ganglion cells promotes axon regeneration in an optic nerve crush mouse model. To investigate the contribution of Dnmt3a to DNA methylation and axon regeneration, retinal ganglion cells isolated from control and retinal ganglion cell-specific Dnmt3a knockout mice at 2 days post-crush were applied for whole genome bisulfite-seq analysis by Illumina NovaSeq 6000.
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| Overall design |
Retinal ganglion cells were isolated by magnetic beads from 2 cKO and 2 sex-matched control mice with optic nerve crush. Genomic DNA was extracted from the retinal ganglion cells and pooled for each group. Methylation profiling by high throughput sequencing was performed for all samples.
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| Contributor(s) |
Kriukov E, Tai WL, Baranov P, Chen DF |
| Citation(s) |
38014168 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| U24 EY029893 |
Retinal Ganglion Cell Replacement in Clinically Relevant Models of Optic Neuropathy |
VANDERBILT UNIVERSITY MEDICAL CENTER |
Petr Baranov |
| R01 EY031696 |
Innate and Adaptive Immunity in the Pathogenesis of Glaucoma |
THE SCHEPENS EYE RESEARCH INSTITUTE, INC. |
Dong Feng Chen |
| R21 EY033882 |
Histone and DNA methyltransferases in optic nerve regeneration |
THE SCHEPENS EYE RESEARCH INSTITUTE, INC. |
Dong Feng Chen |
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| Submission date |
Apr 05, 2023 |
| Last update date |
Dec 15, 2023 |
| Contact name |
Emil Kriukov |
| E-mail(s) |
emilprmu@gmail.com
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| Organization name |
Schepens Eye Research Institute
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| Street address |
20 Staniford St
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| City |
Boston |
| ZIP/Postal code |
02114 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA952614 |
