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| Status |
Public on May 25, 2023 |
| Title |
Old and newly synthesized histones are asymmetrically distributed in Drosophila intestinal stem cell divisions |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report that preexisting (old) and newly synthesized (new) histones H3 and H4 are asymmetrically partitioned during the division of Drosophila intestinal stem cells (ISCs). Furthermore, the inheritance patterns of old and new H3 and H4 in postmitotic cell pairs correlate with distinct expression patterns of Delta, an important cell fate gene. To understand the biological significance of this phenomenon, we expressed a mutant H3T3A to compromise asymmetric histone inheritance. Under this condition, we observe an increase in Delta‐symmetric cell pairs and overpopulated ISC‐like, Delta‐positive cells. Single‐cell RNA‐seq assays further indicate that H3T3A expression compromises ISC differentiation. Together, our results indicate that asymmetric histone inheritance potentially contributes to establishing distinct cell identities in a somatic stem cell lineage, consistent with previous findings in Drosophila male germline stem cells.
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| Overall design |
H3 wild-type- or H3T3A-expressing intestines were dissected, and the tissue samples were analyzed using scRNA-seq.
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| Contributor(s) |
Zion EH, Ringwalt D, Rinaldi K, Kahney EW, Li Y, Chen X |
| Citation(s) |
37255015 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F31 DK122702 |
The Role of Asymmetric Histone Inheritance in Establishing Distinct Cell Identities |
JOHNS HOPKINS UNIVERSITY |
Emily Hope Zion |
| F31 GM122339 |
Investigating epigenetic inheritance patterns and mechanisms during asymmetric division of Drosophila female germline stem cells |
JOHNS HOPKINS UNIVERSITY |
Elizabeth Willa Kahney |
| R35 GM127075 |
Study epigenetic inheritance during development and across generations using multiple model |
JOHNS HOPKINS UNIVERSITY |
Xin Chen |
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| Submission date |
Mar 27, 2023 |
| Last update date |
Aug 29, 2023 |
| Contact name |
Daniel Ringwalt |
| E-mail(s) |
dringwa1@jhu.edu
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| Organization name |
Johns Hopkins University
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| Department |
Biology
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| Lab |
Xin Chen Lab
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| Street address |
3400N Charles St
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21218 |
| Country |
USA |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA949208 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE228292_RAW.tar |
46.9 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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