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| Status |
Public on Sep 26, 2023 |
| Title |
SARS-CoV-2 infection establishes an enhanced, stable, and age-independent CD8+ T cell response against a dominant nucleocapsid epitope using highly restricted TCRs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In vitro antigenic challenge identified N222-230 (LLLDRLNQL referred to as LLL) as a dominant epitope that elicited the greatest response (number of responders and magnitude of expansion) from both convalescent and uninfected donors. Analysis of scTCRseq of LLL-specific CD8+ T cells using scTCRseq revealed highly restricted V-gene usage (TRAV12-2) with limited CDR3 motifs, which was supported by structural characterization of a TCR–LLL–HLA-A2 complex. Lastly, transcriptome analysis of CD8+ T cells between expanders and non-expanders identified increased gene expressions associated with differentiation of CD8+ T cells from non- expanders which are shared across the different subsets in a TCR-independent manner. These results show that SARS-CoV-2 infection resulted in significantly enhanced proliferation of LLL-specific CD8+ T cells and suggest that an altered transcriptome is the underlying mechanism controlling activation-induced expansion of LLL-specific CD8+ T cells.
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| Overall design |
Single Cell sequencing of TCRs and Gene Expression
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| Contributor(s) |
Weng N, Lu J, Choy C, Chen J |
| Citation(s) |
37872153 |
| |
| Submission date |
Mar 22, 2023 |
| Last update date |
Dec 26, 2023 |
| Contact name |
Joseph Ruixin Chen |
| E-mail(s) |
josephchenmn@gmail.com
|
| Phone |
6123568857
|
| Organization name |
National Institute of Health
|
| Department |
National Institute of Aging
|
| Lab |
LMBI
|
| Street address |
R323 Pegram 1340 Campus Drive
|
| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27708 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA947597 |
