|
| Status |
Public on Aug 07, 2023 |
| Title |
Type I IFN Signaling Drives Responsiveness to Short Course Radiotherapy in a Murine Model of Rectal Cancer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Specific cell types from orthotopic murine rectal tumors were sequenced to explore the effect that short course radiotherapy has on the immune response.
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| |
|
| Overall design |
MC38-luc tumor cells were orthotopically implanted in the rectum of C57BL6 female mice. Tumors were irradiated using a small animal irradiator from days 9-13 post tumor implantation. Tumors were treated with 5Gy radiation therapy for 5 consecutive daily fractions. Some mice were treated with anti-IFNAR blocking antibody. Tumors were harvested on day 14 and individual cell populations includining CD8 T cells (CD45+ CD8+), cancer associated fibroblasts (CD45-, CD31- Podoplanin+, PDGFRa+, Ly6c+), and tumor cells (GFP+) were sorted and processed for RNA sequencing.
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| |
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| Contributor(s) |
Uccello T, Gerber S |
| Citation(s) |
37090639, 37495596 |
| |
| Submission date |
Mar 20, 2023 |
| Last update date |
Aug 08, 2023 |
| Contact name |
Scott Gerber |
| E-mail(s) |
scott_gerber@urmc.rochester.edu
|
| Organization name |
University of Rochester
|
| Department |
Microbiology and Immunology
|
| Lab |
Gerber
|
| Street address |
601 Elmwood Ave
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (56)
|
|
| Relations |
| BioProject |
PRJNA946734 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE227738_deSeq2_IFNabR_vs_IgG.txt.gz |
700.3 Kb |
(ftp)(http) |
TXT |
| GSE227738_deSeq2_NONRESPONDERS_vs_UNTREATED.txt.gz |
739.1 Kb |
(ftp)(http) |
TXT |
| GSE227738_deSeq2_RESPONDERS_vs_NONRESPONDERS.txt.gz |
743.3 Kb |
(ftp)(http) |
TXT |
| GSE227738_deSeq2_RESPONDERS_vs_UNTREATED.txt.gz |
783.3 Kb |
(ftp)(http) |
TXT |
| GSE227738_deSeq2_d14_CAF_1-5_vs_d14_CAF_A.txt.gz |
775.0 Kb |
(ftp)(http) |
TXT |
| GSE227738_deSeq2_d14_CAF_1-5_vs_d14_CAF_B.txt.gz |
751.3 Kb |
(ftp)(http) |
TXT |
| GSE227738_deSeq2_d14_CAF_A_vs_d14_CAF_B.txt.gz |
737.3 Kb |
(ftp)(http) |
TXT |
| GSE227738_deSeq2_d14_tumor_1-5_vs_d14_tumor_A.txt.gz |
753.9 Kb |
(ftp)(http) |
TXT |
| GSE227738_deSeq2_d14_tumor_1-5_vs_d14_tumor_B.txt.gz |
711.0 Kb |
(ftp)(http) |
TXT |
| GSE227738_deSeq2_d14_tumor_A_vs_d14_tumor_B.txt.gz |
616.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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