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| Status |
Public on Mar 15, 2023 |
| Title |
Cis-regulatory control of transcriptional timing and noise in response to estrogen [PRO-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Cis-Regulatory Elements (CREs) precisely control transcription levels, temporal dynamics, and cell-cell variation - often referred to as transcriptional noise. However, the combination of regulatory proteins and epigenetic features necessary to control different transcription attributes is not fully understood. Here, single-cell RNA-seq (scRNA-seq) is conducted during a time course of estrogen treatment to identify genomic predictors of expression timing and noise. We find that multiple active enhancers predict faster temporal responses. Synthetic modulation of enhancer activity verifies that activating enhancers accelerates expression responses, while inhibiting enhancers results in a delayed response. Noise is controlled by a balance of promoter and enhancer activity. Active promoters are found at genes with low noise levels, whereas active enhancers are associated with high noise. Finally, we observe that co-expression across single cells is an emergent property dependent on chromatin looping, timing, and noise levels. Overall, our results indicate a fundamental tradeoff between a gene’s ability to quickly respond to incoming signals and maintain low variation across cells.
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| Overall design |
PRO-seq is used to map nascent transcripts in the context of estrogen treatment in endometrial cancer (Ishikawa) and breast cancer (T-47D) cells.
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| Contributor(s) |
Ginley-Hidinger M, Gertz J |
| Citation(s) |
38663407 |
| |
| Submission date |
Mar 13, 2023 |
| Last update date |
May 30, 2024 |
| Contact name |
Jason Gertz |
| E-mail(s) |
jay.gertz@hci.utah.edu
|
| Organization name |
University of Utah
|
| Lab |
Gertz
|
| Street address |
1950 Cir of Hope Dr
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE227245 |
Cis-regulatory control of transcriptional timing and noise in response to estrogen |
|
| Relations |
| BioProject |
PRJNA944219 |
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