|
| Status |
Public on Dec 16, 2023 |
| Title |
RNA-Seq analysis Ezh2 knockdown effect on vascular smooth muscle cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Vascular smooth muscle cells (VSMCs) phenotype switch has been thought to be critical to the development of thoracic aneurysm/dissection. To investigate the function EZH2 in the regulation of VSMCs phenotype switch, we established mouse vascular smooth muscle cells in which each target gene has been knocked down by siRNA.
|
| |
|
| Overall design |
VSMCs isolated and cultured from mouse aorta were siEzh2 or control (n=3 for each group) for 48 hours. Total RNA was isolated using Trizol reagent (Invitrogen, USA). Agilent 2100 Bioanalyzer (Agilent Technologies, USA) was used to evaluate the RNA quality.
|
| |
|
| Contributor(s) |
Yu W, Leng S, Xue S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 12, 2023 |
| Last update date |
Dec 18, 2023 |
| Contact name |
Shuai Leng |
| E-mail(s) |
lengshuai@tmu.edu.cn
|
| Organization name |
Central Hospital Affiliated to Shandong First Medical University
|
| Street address |
105 Jiefang Road
|
| City |
Jinan, Shandong |
| ZIP/Postal code |
250000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA943510 |
Less...
More...