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| Status |
Public on May 08, 2023 |
| Title |
Lp(a) and 17K recombinant apo(a) treatment of THP-1 macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Lp(a) and its distinguishing apolipoprotein constituent apo(a) have been shown to elicit proinflammatory responses from monocytes and macrophages. To obtain a global picture of the effect of Lp(a) and apo(a) on the transcriptome of these cells, we treated THP-1 macrophages with 250 nM Lp(a) or apo(a) for 6 hours, harvested RNA from the cells, and then performed RNA-seq using an Illumina NextSeq instrument. Analysis of the data using GO and KEGG strategies revealed a series of proinflammatory genes upregulated by apo(a) and even more dramatically by Lp(a).
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| Overall design |
Duplicate wells of THP-1 macrophages were treated for 6 hours with 250 nM Lp(a) or recombinant 17-kringle apo(a), or vehicle alone (phosphate-buffered saline).
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| Web link |
https://www.frontiersin.org/articles/10.3389/fcvm.2023.1130162/full#supplementary-material
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| |
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| Contributor(s) |
Borrelli M, Carter D, Boffa M, Koschinsky M |
| Citation(s) |
37293282 |
| |
| Submission date |
Mar 06, 2023 |
| Last update date |
Aug 08, 2023 |
| Contact name |
Michael Boffa |
| E-mail(s) |
mboffa@uwo.ca
|
| Organization name |
The University of Western Ontario
|
| Department |
Biochemistry
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| Lab |
Room 4245A Robarts Research Institute
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| Street address |
1151 Richmond Street North
|
| City |
London |
| State/province |
ON |
| ZIP/Postal code |
N6A 5B7 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA941338 |
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