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Series GSE226759 Query DataSets for GSE226759
Status Public on May 08, 2023
Title Lp(a) and 17K recombinant apo(a) treatment of THP-1 macrophages
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Lp(a) and its distinguishing apolipoprotein constituent apo(a) have been shown to elicit proinflammatory responses from monocytes and macrophages. To obtain a global picture of the effect of Lp(a) and apo(a) on the transcriptome of these cells, we treated THP-1 macrophages with 250 nM Lp(a) or apo(a) for 6 hours, harvested RNA from the cells, and then performed RNA-seq using an Illumina NextSeq instrument. Analysis of the data using GO and KEGG strategies revealed a series of proinflammatory genes upregulated by apo(a) and even more dramatically by Lp(a).
 
Overall design Duplicate wells of THP-1 macrophages were treated for 6 hours with 250 nM Lp(a) or recombinant 17-kringle apo(a), or vehicle alone (phosphate-buffered saline).
Web link https://www.frontiersin.org/articles/10.3389/fcvm.2023.1130162/full#supplementary-material
 
Contributor(s) Borrelli M, Carter D, Boffa M, Koschinsky M
Citation(s) 37293282
Submission date Mar 06, 2023
Last update date Aug 08, 2023
Contact name Michael Boffa
E-mail(s) mboffa@uwo.ca
Organization name The University of Western Ontario
Department Biochemistry
Lab Room 4245A Robarts Research Institute
Street address 1151 Richmond Street North
City London
State/province ON
ZIP/Postal code N6A 5B7
Country Canada
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (9)
GSM7083235 A1
GSM7083236 A14
GSM7083237 A6
Relations
BioProject PRJNA941338

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Supplementary file Size Download File type/resource
GSE226759_Partek_Koschinsky_9_Normalization_Normalized_counts.txt.gz 649.5 Kb (ftp)(http) TXT
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