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| Status |
Public on Jul 06, 2023 |
| Title |
p53 inhibitor iASPP is an unexpected suppressor of KRAS and inflammation driven pancreatic cancer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the transcriptomic effects of iASPP deletion or p53 mutation in the KRASG12D background Pdx1-Cre was used to delete iASPP in oncogenic KRASG12D expressing (KC) background to generate KC;iASPPΔ8/Δ8 mice. KC and KC;iASPPΔ8/Δ8 mice were then crossed with mice containing a p53R172H allele (equivalent to human p53 hotspot mutation R175H) to generate KPC and KPC;iASPPΔ8/Δ8 cohorts. Primary cell cultures were then derived from pancreatic ductal adenocarcinoma tissue for RNA-seq.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for KC, KC;iASPPΔ8/Δ8,KC, and KC;iASPPΔ8/Δ8 mouse cell lines.
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| |
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| Contributor(s) |
Miller P, Owen R, Amess R, Lu X |
| Citation(s) |
37270580 |
| |
| Submission date |
Mar 03, 2023 |
| Last update date |
Jul 06, 2023 |
| Contact name |
Xin Lu |
| E-mail(s) |
xin.lu@ludwig.ox.ac.uk
|
| Phone |
+44 1865 617507
|
| Organization name |
University of Oxford
|
| Department |
Ludwig Institute for Cancer Research
|
| Street address |
Old Road Campus
|
| City |
Oxford |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX3 7DQ |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA940781 |
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