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| Status |
Public on Feb 23, 2023 |
| Title |
NGS analysis of T-ALL cell lines treated with mTORC1 inhibitor |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We recentlly showed that everolimus, a mTORC1 inhibitor increases reactive oxygen species (ROS) levels, decreases the levels of NADPH and of glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the pentose phosphate pathway (PPP), and induces apoptosis of T-ALL cells.
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| Overall design |
Jurkat, TALL-1 and CEM cells lines were treated with 10 μM everolimus or drug vehicle after for 24 hours. Then we collected cells and extracted the RNA to perform the RNASEq analysis.
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| Contributor(s) |
Urso L, Corradin A, Silic-Benussi M, Ciminale V |
| Citation(s) |
36978873 |
| |
| Submission date |
Feb 21, 2023 |
| Last update date |
Apr 04, 2023 |
| Contact name |
Loredana Urso |
| E-mail(s) |
loredana.urso@unipd.it
|
| Organization name |
Università degli studi di Padova
|
| Department |
DISCOG
|
| Street address |
gattamelata 64
|
| City |
pADOVA |
| ZIP/Postal code |
35128 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA937271 |
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