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| Status |
Public on Apr 25, 2023 |
| Title |
A single-cell RNA sequencing atlas of circulating leukocytes from healthy and osteosarcoma affected dogs |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used single-cell RNA sequencing to characterize the heterogeneity of circulating leukocytes in dogs, then employed the dataset to investigate how primary osteosarcoma (OS) tumors impacted circulating leukocytes.
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| |
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| Overall design |
The complete dataset consists of circulating leukocytes from 7 healthy dogs and 10 OS affected dogs. The final processed Seurat objects are provided as 'final_dataSet_H.rds' (Healthy only) and 'final_dataSet_HvO.rds' (Healthy and osteosarcoma PBMCs). Each major immune cell type subset is provided where 'cytotoxic.rds' = CD8/NK cell, 'helper.rds' = CD4 T cells, 'myeloid.rds' = Myeloid, and 'bcell.rds' = B cells. All R code used to complete the analysis can be found at: https://github.com/dyammons/Canine_Leukocyte_scRNA.
|
| Web link |
https://www.frontiersin.org/articles/10.3389/fimmu.2023.1162700/full
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| |
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| Contributor(s) |
Ammons DT, Dow S |
| Citation(s) |
37275879 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| U01 CA224182 |
Optimizing Novel Immunotherapy Combinations Targeting the Tumor Microenvironment in Canine Spontaneous Osteosarcoma |
COLORADO STATE UNIVERSITY |
Steven W. Dow |
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| |
| Submission date |
Feb 19, 2023 |
| Last update date |
Jul 03, 2023 |
| Contact name |
Dylan T Ammons |
| E-mail(s) |
Dylan.Ammons@colostate.edu
|
| Phone |
4846519191
|
| Organization name |
Colorado State University
|
| Department |
MIP
|
| Street address |
200 W Lake St
|
| City |
Fort Collins |
| State/province |
CO |
| ZIP/Postal code |
80523 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL25760 |
Illumina NovaSeq 6000 (Canis lupus familiaris) |
|
| Samples (17)
|
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| Relations |
| BioProject |
PRJNA936588 |
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