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Series GSE225547 Query DataSets for GSE225547
Status Public on Feb 19, 2023
Title Reverse Transcription quantitative PCR (RT-q PCR) analysis of osteogenic-related markers for osteogenic differentiation from human periodontal ligamen stem cells
Organism Homo sapiens
Experiment type Expression profiling by RT-PCR
Summary We pre-treated human periodontal ligament stem cells (hPDLSCs) under Iinterleukin-6 (IL-6) in osteogenic inductive medium (OM) on osteogenic differentiation potential and investigate the specific signaling pathway by clarifying with several antagonists (Dkk-1, SP600125, SB431542, and DAPT) and siRNA (siWnt2B, siWnt10B, and siWnt5A) to identify the differences in the expression pattern of osteogenic related markers by reverse transcription quantitative polymerase chain reaction (RT-qPCR).
 
Overall design qPCR gene expression profiling of differentiation cells compared to undifferentiated cells.
 
Contributor(s) Sawangmake C, Purwaningrum M
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Submission date Feb 17, 2023
Last update date Feb 20, 2023
Contact name Medania Purwaningrum
E-mail(s) medania@mail.ugm.ac.id
Phone +6281802714777
Organization name Faculty of Veterinary Science
Department Pharmacology
Lab Veterinary Stem Cells and Bioengineering Innovation Center
Street address Henri Dunant
City Pathumwan
State/province Bangkok
ZIP/Postal code 10330
Country Thailand
 
Platforms (1)
GPL33139 VSCBIC hPDLSCs CF96TM real-time PCR detection system
Samples (40)
GSM7050170 Control Undifferentiation Cell 1
GSM7050171 Control Undifferentiation Cell 2
GSM7050172 Control Undifferentiation Cell 3
Relations
BioProject PRJNA936165

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE225547_fold-change.txt.gz 2.8 Kb (ftp)(http) TXT
GSE225547_non-normalized.txt.gz 3.2 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record
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