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| Status |
Public on Apr 18, 2023 |
| Title |
Intrinsic TGF-b Signaling Attenuates Proximal Tubule Mitochondrial Injury and Inflammation in Chronic Kidney Disease |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate how TGF-b affects proximal tubule cell metabolism and response to injury, we generated conditional immortalized mouse PT cells and deleted the TGF-beta Receptor II in vitro using adeno-cre
We then performed gene expression profiling analysis using data obtained from RNA-seq of WT cells (PT cre-) and KO cells (PTcrex2) treated or not with H2O2 in serum free media (4 conditions in total)
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| |
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for PT cre- and PT crex2 at baseline and under stress condition (H2O2 and serum free media)
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| |
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| Contributor(s) |
Nlandu Khodo S, Tan G, Leary P, Rehrauer H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 17, 2023 |
| Last update date |
Apr 22, 2023 |
| Contact name |
Genome Informatics |
| E-mail(s) |
fgcz.gi@gmail.com
|
| Organization name |
ETH ZURICH / University of Zurich
|
| Department |
Functional Genomics Center Zurich (FGCZ)
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA936155 |
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