|
| Status |
Public on Jan 18, 2024 |
| Title |
Overexpression of FOXO1 enhances CAR T cell polyfunctionality, metabolic fitness and efficacy against solid tumors [Mouse RNAseq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Investigating the effect on CAR T cell phenotype by overexpressing FOXO1
|
| |
|
| Overall design |
Murine C57BL/6 splenocytes were transduced to express a human HER2 directed CAR and a constitutively active variant of Foxo1 (Foxo1-ADA) or empty vector. CAR T cells were cultured with a CAR agonistic antibody for the HER2 CAR over 72 hours, purified and analyzed with 3' RNA seq.
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| |
|
| Contributor(s) |
Chan J, Beavis P, Sek K |
| Citation(s) |
38600376 |
| |
| Submission date |
Feb 17, 2023 |
| Last update date |
May 08, 2024 |
| Contact name |
Kevin C sek |
| E-mail(s) |
kevin.sek@petermac.org
|
| Phone |
0432960331
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Department |
Cancer Immunology
|
| Lab |
Beavis Lab
|
| Street address |
305 Grattan Street
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3000 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (8)
|
| GSM7049826 |
Unstimulated_NGFR_control_rep1 (RNA-seq) |
| GSM7049827 |
Unstimulated_NGFR_control_rep2 (RNA-seq) |
| GSM7049828 |
Stimulated_NGFR_control_rep1 (RNA-seq) |
|
| This SubSeries is part of SuperSeries: |
| GSE225527 |
Overexpression of FOXO1 enhances CAR T cell polyfunctionality, metabolic fitness and efficacy against solid tumors |
|
| Relations |
| BioProject |
PRJNA936117 |
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