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| Status |
Public on Feb 16, 2023 |
| Title |
Influenza A virus modulation of Streptococcus pneumoniae infection using ex vivo transcriptomics in a human primary lung epithelial cell |
| Organisms |
Streptococcus pneumoniae; Homo sapiens; Influenza A virus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Deep sequencing of cDNA from Streptococcus pneumoniae EF3030 bacteria and normalized human broncial epithelial cells (nHBEC), alone, together, and after coincubation with Influenza A virus pH1N1.
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| Overall design |
Total RNA was isolated from EF3030 and nHBEC, alone and after co-culture with pH1N1 over time. Ribosomal RNA was depleted and the remaining RNA was reverse-transcribed into a cDNA library for Illumina sequencing. Reads were mapped to the EF3030,and the human genome.
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| Contributor(s) |
Harrod KS, Tipper JL, Orihuela CJ, D'Mello A, Lane JR, MartÃnez E, Roussey HN, Nadendla S, Tettelin H |
| Citation(s) |
36778321 |
| |
| Submission date |
Feb 11, 2023 |
| Last update date |
May 18, 2023 |
| Contact name |
Suvarna Nadendla |
| Organization name |
University of Maryland School of Medicine
|
| Department |
Institute for Genome Sciences
|
| Street address |
670 W.Baltimore Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
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| Platforms (5)
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| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL28680 |
Illumina NovaSeq 6000 (Streptococcus pneumoniae) |
| GPL33118 |
Illumina NovaSeq 6000 (Homo sapiens; Streptococcus pneumoniae) |
| GPL33119 |
Illumina NovaSeq 6000 (Homo sapiens; Influenza A virus) |
| GPL33120 |
Illumina NovaSeq 6000 (Homo sapiens; Influenza A virus; Streptococcus pneumoniae) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA933933 |
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